PD Dr. Anna Marsano

Reid, G., Cerino, G., Melly, L., Fusco, D., Zhang, C., Reuthebuch, O., Milan, G., & Marsano, A. (2025). Harnessing the angiogenic potential of adipose-derived stromal vascular fraction cells with perfusion cell seeding [Journal-article]. Stem Cell Research &Amp; Therapy, 16(1). https://doi.org/10.1186/s13287-025-04286-6

Wittner, R., Holub, P., Mascia, C., Frexia, F., Müller, H., Plass, M., Allocca, C., Betsou, F., Burdett, T., Cancio, I., Chapman, A., Chapman, M., Courtot, M., Curcin, V., Eder, J., Elliot, M., Exter, K., Goble, C., Golebiewski, M., et al. (2024). Toward a common standard for data and specimen provenance in life sciences. Learning Health Systems, 8(1). https://doi.org/10.1002/lrh2.10365

Borisov, Vladislav, Gili Sole, Laia, Reid, Gregory, Milan, Giulia, Hutter, Gregor, Grapow, Martin, Eckstein, Friedrich Stefan, Isu, Giuseppe, & Marsano, Anna. (2023). Upscaled Skeletal Muscle Engineered Tissue with In Vivo Vascularization and Innervation Potential. Bioengineering, 10(7). https://doi.org/10.3390/bioengineering10070800

Gabetti, S., Sileo, A., Montrone, F., Putame, G., Audenino, A. L., Marsano, A., & Massai, D. (2023). Versatile electrical stimulator for cardiac tissue engineering—Investigation of charge-balanced monophasic and biphasic electrical stimulations. Frontiers in Bioengineering and Biotechnology, 10. https://doi.org/10.3389/fbioe.2022.1031183

Fusco D, Meissner F, Podesser BK, Marsano A, Grapow M, Eckstein F, & Winkler B. (2022). Small-diameter bacterial cellulose-based vascular grafts for coronary artery bypass grafting in a pig model. Frontiers in Cardiovascular Medicine, 9, 881557. https://doi.org/10.3389/fcvm.2022.881557

Pisanu A., Reid G., Fusco D., Sileo A., Robles Diaz D., Tarhini H., Putame G., Massai D., Isu G., & Marsano A. (2022). Bizonal cardiac engineered tissues with differential maturation features in a mid-throughput multimodal bioreactor. iScience, 25(5). https://doi.org/10.1016/j.isci.2022.104297

Anna Marsano. (2022). Towards a Common Standard for Data and Specimen Provenance in Life Sciences. Learning Health Systems.

Züger, Fabian, Marsano, Anna, Poggio, Martino, & Gullo, Maurizio R. (2022). Nanocomposites in 3D Bioprinting for Engineering Conductive and Stimuli-Responsive Constructs Mimicking Electrically Sensitive Tissue. Advanced NanoBiomed Research, 2(2), 2100108. https://doi.org/10.1002/anbr.202100108

Mainardi, Andrea, Carminati, Francesca, Ugolini, Giovanni Stefano, Occhetta, Paola, Isu, Giuseppe, Robles Diaz, Diana, Reid, Gregory, Visone, Roberta, Rasponi, Marco, & Marsano, Anna. (2021). A dynamic microscale mid-throughput fibrosis model to investigate the effects of different ratios of cardiomyocytes and fibroblasts. Lab on a Chip, 21(21), 4177–4195. https://doi.org/10.1039/d1lc00092f

Mytsyk M, Cerino G., Reid G, Sole LG, Eckstein F.S., Santer D, & Marsano A. (2021). Long-term severe in vitro hypoxia exposure enhances the vascularization potential of human adipose tissue-derived stromal vascular fraction cell engineered tissues. International Journal of Molecular Sciences, 22(15). https://doi.org/10.3390/ijms22157920

Koechlin L, Isu G, Borisov V, Robles Diaz D, Eckstein FS, Marsano A, & Reuthebuch O. (2021). Impact on mechanical properties of 10 versus 20 minute treatment of human pericardium with glutaraldehyde in ozaki procedure. Annals of Thoracic and Cardiovascular Surgery, 27(4), 273–277. https://doi.org/10.5761/atcs.nm.20-00125

Reid G, Magarotto F, Marsano A, & Pozzobon M. (2020). Next stage approach to tissue engineering skeletal muscle. Bioengineering, 7(4), 1–30. https://doi.org/10.3390/bioengineering7040118

Massai D, Pisani G, Isu G, Rodriguez Ruiz A, Cerino G, Galluzzi R, Pisanu A, Tonoli A, Bignardi C, Audenino AL, Marsano A, & Morbiducci U. (2020). Bioreactor Platform for Biomimetic Culture and in situ Monitoring of the Mechanical Response of in vitro Engineered Models of Cardiac Tissue. Frontiers in Bioengineering and Biotechnology, 8, 733. https://doi.org/10.3389/fbioe.2020.00733

Isu G, Robles Diaz D, Grussenmeyer T, Gaudiello E, Eckstein F., Brink M, & Marsano A. (2020). Fatty acid-based monolayer culture to promote in vitro neonatal rat cardiomyocyte maturation. Biochimica et Biophysica Acta - Molecular Cell Research, 1867(3). https://doi.org/10.1016/j.bbamcr.2019.118561

Melly, Ludovic, Grosso, Andrea, Stanciu Pop, Claudia, Yu-Hsuan, Chu, Nollevaux, Marie-Cécile, Schachtrup, Christian, Marsano, Anna, Di Maggio, Nunzia, Rondelet, Benoît, & Banfi, Andrea. (2020). Fibrin hydrogels promote scar formation and prevent therapeutic angiogenesis in the heart. Journal of tissue engineering and regenerative medicine, 14(10), 1513–1523. https://doi.org/10.1002/term.3118

Luca Koechlin, Giuseppe Isu, Vladislav Borisov, Diana Robles Diaz, Friedrich Eckstein, Anna Marsano, & Oliver Reuthebuch. (2020). Impact on mechanical properties of 10 versus 20 minutes treatment of human pericardium with gluteraldehde in OZAKY procedure. Annals of Thoracic and Cardiovascular Surgery.

Isu G, Morbiducci U, De Nisco G, Kropp C, Marsano A, Deriu MA, Zweigerdt R, Audenino A, & Massai D. (2019). Modeling methodology for defining a priori the hydrodynamics of a dynamic suspension bioreactor. Application to human induced pluripotent stem cell culture. Journal of Biomechanics, 94, 99–106. https://doi.org/10.1016/j.jbiomech.2019.07.021

Mytsyk, Myroslava, Isu, Giuseppe, Cerino, Giulia, Grapow, Martin T. R., Eckstein, Friedrich S., & Marsano, Anna. (2019). Paracrine potential of adipose stromal vascular fraction cells to recover hypoxia-induced loss of cardiomyocyte function. Biotechnology and Bioengineering, 116(1), 132–142. https://doi.org/10.1002/bit.26824

Occhetta P, Isu G, Lemme M, Conficconi C, Oertle P, Räz C, Visone R, Cerino G, Plodinec M, Rasponi M, & Marsano A. (2018). A three-dimensional: In vitro dynamic micro-tissue model of cardiac scar formation. Integrative Biology (United Kingdom), 10(3), 174–183. https://doi.org/10.1039/c7ib00199a

Melly, Ludovic, Cerino, Giulia, Frobert, Aurélien, Cook, Stéphane, Giraud, Marie-Noëlle, Carrel, Thierry, Tevaearai Stahel, Hendrik T., Eckstein, Friedrich, Rondelet, Benoît, Marsano, Anna, & Banfi, Andrea. (2018). Myocardial infarction stabilization by cell-based expression of controlled Vascular Endothelial Growth Factor levels. Journal of cellular and molecular medicine, 22(5), 2580–2591. https://doi.org/10.1111/jcmm.13511

Cerino G, Gaudiello E, Muraro MG, Eckstein F, Martin I, Scherberich A, & Marsano A. (2017). Engineering of an angiogenic niche by perfusion culture of adipose-derived stromal vascular fraction cells. Scientific Reports, 7(1), 14252. https://doi.org/10.1038/s41598-017-13882-3

Staubli, Sebastian Manuel, Cerino, Giulia, Gonzalez De Torre, Israel, Alonso, Matilde, Oertli, Daniel, Eckstein, Friedrich, Glatz, Katharina, Rodríguez Cabello, José Carlos, & Marsano, Anna. (2017). Control of angiogenesis and host response by modulating the cell adhesion properties of an Elastin-Like Recombinamer-based hydrogel. Biomaterials, 135, 30–41. https://doi.org/10.1016/j.biomaterials.2017.04.047

Gaudiello, Emanuele, Melly, Ludovic, Cerino, Giulia, Boccardo, Stefano, Jalili-Firoozinezhad, Sasan, Xu, Lifen, Eckstein, Friedrich, Martin, Ivan, Kaufmann, Beat A., Banfi, Andrea, & Marsano, Anna. (2017). Scaffold Composition Determines the Angiogenic Outcome of Cell-Based Vascular Endothelial Growth Factor Expression by Modulating Its Microenvironmental Distribution. Advanced healthcare materials, 6(24), 1700600. https://doi.org/10.1002/adhm.201700600

Medeiros Da Cunha, Carolina M., Perugini, Valeria, Bernegger, Petra, Centola, Matteo, Barbero, Andrea, Guildford, Anna L., Santin, Matteo, Banfi, Andrea, Martin, Ivan, & Marsano, Anna. (2017). Vascular Endothelial Growth Factor Sequestration Enhances In Vivo Cartilage Formation. International journal of molecular sciences, 18(11), 2478. https://doi.org/10.3390/ijms18112478

Mochizuki, Michika, Lorenz, Vera, Ivanek, Robert, Della Verde, Giacomo, Gaudiello, Emanuele, Marsano, Anna, Pfister, Otmar, & Kuster, Gabriela M. (2017). Polo-Like Kinase 2 is Dynamically Regulated to Coordinate Proliferation and Early Lineage Specification Downstream of Yes-Associated Protein 1 in Cardiac Progenitor Cells. Journal of the American Heart Association, 6(10), e005920. https://doi.org/10.1161/jaha.117.005920

Jalili-Firoozinezhad, Sasan, Rajabi-Zeleti, Sareh, Marsano, Anna, Aghdami, Nasser, & Baharvand, Hossein. (2016). Influence of decellularized pericardium matrix on the behavior of cardiac progenitors. Journal of Applied Polymer Science, 133(14). https://doi.org/10.1002/app.43255

Boccardo, Stefano, Gaudiello, Emanuele, Melly, Ludovic, Cerino, Giulia, Ricci, Davide, Martin, Ivan, Eckstein, Friedrich, Banfi, Andrea, & Marsano, Anna. (2016). Engineered mesenchymal cell-based patches as controlled VEGF delivery systems to induce extrinsic angiogenesis. Acta Biomaterialia, 42, 127–135. https://doi.org/10.1016/j.actbio.2016.07.041

Cerino, Giulia, Gaudiello, Emanuele, Grussenmeyer, Thomas, Melly, Ludovic, Massai, Diana, Banfi, Andrea, Martin, Ivan, Eckstein, Friedrich, Grapow, Martin, & Marsano, Anna. (2016). Three dimensional multi-cellular muscle-like tissue engineering in perfusion-based bioreactors. Biotechnology and Bioengineering, 113(1), 226–236. https://doi.org/10.1002/bit.25688

Marsano, Anna, Conficconi, Chiara, Lemme, Marta, Occhetta, Paola, Gaudiello, Emanuele, Votta, Emiliano, Cerino, Giulia, Redaelli, Alberto, & Rasponi, Marco. (2016). Beating heart on a chip: a novel microfluidic platform to generate functional 3D cardiac microtissues. Lab on a Chip, 16(3), 599–610. https://doi.org/10.1039/c5lc01356a

Marsano, Anna, Medeiros da Cunha, Carolina M., Ghanaati, Shahram, Gueven, Sinan, Centola, Matteo, Tsaryk, Roman, Barbeck, Mike, Stuedle, Chiara, Barbero, Andrea, Helmrich, Uta, Schaeren, Stefan, Kirkpatrick, James C., Banfi, Andrea, & Martin, Ivan. (2016). Spontaneous In Vivo Chondrogenesis of Bone Marrow-Derived Mesenchymal Progenitor Cells by Blocking Vascular Endothelial Growth Factor Signaling. Stem Cells Translational Medicine, 5(12), 1730–1738. https://doi.org/10.5966/sctm.2015-0321

Visone, Roberta, Gilardi, Mara, Marsano, Anna, Rasponi, Marco, Bersini, Simone, & Moretti, Matteo. (2016). Cardiac Meets Skeletal: What’s New in Microfluidic Models for Muscle Tissue Engineering. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 21(9), 1128–1149. https://doi.org/10.3390/molecules21091128

Jalili-Firoozinezhad, Sasan, Rajabi-Zeleti, Sareh, Mohammadi, Parvaneh, Gaudiello, Emanuele, Bonakdar, Shahin, Solati-Hashjin, Mehran, Marsano, Anna, Aghdami, Nasser, Scherberich, Arnaud, Baharvand, Hossein, & Martin, Ivan. (2015). Facile fabrication of egg white macroporous sponges for tissue regeneration. Advanced Healthcare Materials, 4(15), 2281–2290. https://doi.org/10.1002/adhm.201500482

Dohle, E., Bischoff, I., Böse, T., Marsano, A., Banfi, A., Unger, R. E., & Kirkpatrick, C. J. (2014). Macrophage-mediated angiogenic activation of outgrowth endothelial cells in co-culture with primary osteoblasts. European Cells and Materials, 27, 149–64; discussion 164–5. https://doi.org/10.22203/ecm.v027a12

Fulco, Ilario, Fulco, Ilario, Miot, Sylvie, Haug, Martin D., Barbero, Andrea, Wixmerten, Anke, Feliciano, Sandra, Wolf, Francine, Jundt, Gernot, Marsano, Anna, Farhadi, Jian, Heberer, Michael, Jakob, Marcel, Schaefer, Dirk J., & Martin, Ivan. (2014). Engineered autologous cartilage tissue for nasal reconstruction after tumour resection: an observational first-in-human trial. The Lancet, 384(9940), 337–346. https://doi.org/10.1016/s0140-6736(14)60544-4

Centola, Matteo, Abbruzzese, Franca, Scotti, Celeste, Barbero, Andrea, Vadalà, Gianluca, Denaro, Vincenzo, Martin, Ivan, Trombetta, Marcella, Rainer, Alberto, & Marsano, Anna. (2013). Scaffold-based delivery of a clinically relevant anti-angiogenic drug promotes the formation of in vivo stable cartilage. Tissue Engineering. Part A, 19(17-18), 1960–1971. https://doi.org/10.1089/ten.tea.2012.0455

Marsano, Anna, Maidhof, Robert, Luo, Jianwen, Fujikara, Kana, Konofagou, Elisa E, Banfi, Andrea, & Vunjak-Novakovic, Gordana. (2013). The effect of controlled expression of VEGF by transduced myoblasts in a cardiac patch on vascularization in a mouse model of myocardial infarction. Biomaterials, 34(2), 393–401. https://doi.org/10.1016/j.biomaterials.2012.09.038

Xie, Lin, Zhang, Nan, Marsano, Anna, Vunjak-Novakovic, Gordana, Zhang, Yanru, & Lopez, Mandi J. (2013). In vitro mesenchymal trilineage differentiation and extracellular matrix production by adipose and bone marrow derived adult equine multipotent stromal cells on a collagen scaffold. Stem Cell Reviews and Reports, 9(6), 858–872. https://doi.org/10.1007/s12015-013-9456-1

Melly, Ludovic, Boccardo, Stefano, Eckstein, Friedrich, Banfi, Andrea, & Marsano, Anna. (2012). Cell and gene therapy approaches for cardiac vascularization. Cells, 1(4), 961–975. https://doi.org/10.3390/cells1040961

Miot S., Marsano A., & Martin I. (2012). Cell-based tissue engineering approaches for disc regeneration (pp. 493–509). Elsevier Ltd. https://doi.org/10.1016/b978-1-84569-986-4.50016-7

Melly, Ludovic F, Marsano, Anna, Frobert, Aurelien, Boccardo, Stefano, Helmrich, Uta, Heberer, Michael, Eckstein, Friedrich S, Carrel, Thierry P, Giraud, Marie-Noëlle, Tevaearai, Hendrik T, & Banfi, Andrea. (2012). Controlled angiogenesis in the heart by cell-based expression of specific vascular endothelial growth factor levels. Human Gene Therapy Methods, 23(5), 346–356. https://doi.org/10.1089/hgtb.2012.032

Zhang, T., Wan, L. Q., Xiong, Z., Marsano, A., Maidhof, R., Park, M., Yan, Y., & Vunjak-Novakovic, G. (2012). Channelled scaffolds for engineering myocardium with mechanical stimulation. Journal of Tissue Engineering and Regenerative Medicine, 6(9), 748–756. https://doi.org/10.1002/term.481

Marsano A., Perugini V., Centola M., Guven S., Banfi A., Meikle S.T., Guildford A.L., Santin M., & Martin I. (2011, December 1). In vivo chondrogenesis by using anti-angiogenic peptides.

Tandon N, Marsano A, Maidhof R, Wan L, Park H, & Vunjak-Novakovic G. (2011). Optimization of electrical stimulation parameters for cardiac tissue engineering. Journal of Tissue Engineering and Regenerative Medicine, 5(6), e115–25. https://doi.org/10.1002/term.377

Maidhof R, Marsano A, Lee EJ, & Vunjak-Novakovic G. (2010). Perfusion seeding of channeled elastomeric scaffolds with myocytes and endothelial cells for cardiac tissue engineering. Biotechnology Progress, 26(2), 565–572. https://doi.org/10.1002/btpr.337

Marsano A., Bernegger P., Helmrich U., Schaeren S., Miot S., Barbero A., Martin I., & Banfi A. (2010). Generation of human nasal chondrocytes engineered to express soluble VEGF receptor-2 for cartilage tissue engineering. 20, 48.

Marsano A, Maidhof R, Wan LQ, Wang Y, Gao J, Tandon N, & Vunjak-Novakovic G. (2010). Scaffold stiffness affects the contractile function of three-dimensional engineered cardiac constructs. Biotechnology progress, 26(5), 1382–1390. https://doi.org/10.1002/btpr.435

Tandon N, Marsano A, Maidhof R, Numata K, Montouri-Sorrentino C, Cannizzaro C, Voldman J, & Vunjak-Novakovic G. (2010). Surface-patterned electrode bioreactor for electrical stimulation. Lab on a Chip, 10(6), 692–700. https://doi.org/10.1039/b917743d

Vunjak-Novakovic, Gordana, Tandon, Nina, Godier, Amandine, Maidhof, Robert, Marsano, Anna, Martens, Timothy P., & Radisic, Milica. (2010). Challenges in cardiac tissue engineering. Tissue engineering. Part B, Reviews, 16(2), 169–187. https://doi.org/10.1089/ten.teb.2009.0352

Wan, L. Q., Zhang, T., Marsano, A., Maidhof, R., & Vunjak-Novakovic, G. (2009). Subpixel texture correlation for contractile behaviors of engineered cardiac tissue. 1275–1276. https://doi.org/10.1115/SBC2009-205851

Zhang, T., Wan, L. Q., Marsano, A., Maidhof, R., Yan, Y., Pan, J., & Vunjak-Novakovic, G. (2009). Chitosan-collagen based channeled scaffold for cardiac tissue engineering. 1191–1192. https://doi.org/10.1115/SBC2009-206639

Tandon N, Cannizzaro C, Chao PH, Maidhof R, Marsano A, Au HT, Radisic M, & Vunjak-Novakovic G. (2009). Electrical stimulation systems for cardiac tissue engineering. Nature Protocols, 4(2), 155–173. https://doi.org/10.1038/nprot.2008.183

Jacobs, Sharone’, Simhaee, David A., Marsano, Anna, Fomovsky, Gregory M., Niedt, George, & Wu, June K. (2009). Efficacy and mechanisms of vacuum-assisted closure (VAC) therapy in promoting wound healing: a rodent model. Journal of Plastic, Reconstructive and Aesthetic Surgery, 62, 1331–1338. https://doi.org/10.1016/j.bjps.2008.03.024

Tandon N, Goh B, Marsano A, Chao PH, Montouri-Sorrentino C, Gimble J, & Vunjak-Novakovic G. (2009). Alignment and elongation of human adipose-derived stem cells in response to direct-current electrical stimulation. Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference, 2009, 6517–6521. https://doi.org/10.1109/IEMBS.2009.5333142

Radisic M, Marsano A, Maidhof R, Wang Y, & Vunjak-Novakovic G. (2008). Cardiac tissue engineering using perfusion bioreactor systems. Nature Protocols, 3(4), 719–738. https://doi.org/10.1038/nprot.2008.40

Marsano, A., Maidhof, R., Tandon, N., Gao, J., Wang, Y., & Vunjak-Novakovic, G. (2008). Engineering of functional contractile cardiac tissues cultured in a perfusion system. 3590–+.

Tandon, N., Marsano, A., Cannizzaro, C., Voldman, J., & Vunjak-Novakovic, G. (2008). Design of Electrical Stimulation Bioreactors for Cardiac Tissue Engineering. 3594–+.

Marsano, A, Millward-Sadler, S J, Salter, D M, Adesida, A, Hardingham, T, Tognana, E, Kon, E, Chiari-Grisar, C, Nehrer, S, Jakob, M, & Martin, I. (2007). Differential cartilaginous tissue formation by human synovial membrane, fat pad, meniscus cells and articular chondrocytes. Osteoarthritis And Cartilage, 15(1), 48–58. https://doi.org/10.1016/j.joca.2006.06.009

Marsano A, Vunjak-Novakovic G, & Martin I. (2006). Towards tissue engineering of meniscus substitutes: Selection of cell source and culture environment. Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings, 2006, 3656–3658. https://doi.org/10.1109/IEMBS.2006.259748

Marsano A, Wendt D, Quinn TM, Sims TJ, Farhadi J, Jakob M, Heberer M, & Martin I. (2006). Bi-zonal cartilaginous tissues engineered in a rotary cell culture system. Biorheology, 43(3-4), 553–560.

Marsano A., Wendt D., Raiteri R., Gottardi R., Dickinson S., Hollander A.P., Wirz D., Daniels A.U., Quinn T.M., Stolz M., & Martin I. (2006). Local compressive and tensile stiffness measured in tissues with regular patterns of hyaline-fibrocartilage regions. 11, 27.

Marsano, Anna, Wendt, David, Raiteri, Roberto, Gottardi, Riccardo, Stolz, Martin, Wirz, Dieter, Daniels, Alma U., Salter, Donald, Jakob, Marcel, Quinn, Thomas M., & Martin, Ivan. (2006). Use of hydrodynamic forces to engineer cartilaginous tissues resembling the non-uniform structure and function of meniscus. Biomaterials, 27(35), 5927–5934. https://doi.org/10.1016/j.biomaterials.2006.08.020

Wendt D, Marsano A, Jakob M, Heberer M, & Martin I. (2003). Oscillating perfusion of cell suspensions through three-dimensional scaffolds enhances cell seeding efficiency and uniformity. Biotechnology and Bioengineering, 84(2), 205–214. https://doi.org/10.1002/bit.10759