Cell Biology (Scheiffele)Head of Research Unit Prof. Dr. Peter Scheiffele, Prof. Dr.OverviewMembersPublicationsProjects & CollaborationsProjects & Collaborations OverviewMembersPublicationsProjects & Collaborations Projects & Collaborations 37 foundShow per page10 10 20 50 10008188 - Impact of autism spectrum disorder-associated mutations on neuronal mRNA translation Research Project | 1 Project MembersImported from Grants Tool 4721840 Molecular analysis of PBMC and brain samples from TSC patients Research Project | 2 Project MembersImported from Grants Tool 4725241 Exploring the proteomes of vulnerable synapse types in Alzheimer’s disease progression Research Project | 1 Project MembersImported from Grants Tool 4717935 Transcription Start Site determinants of Neuronal Identity (TSS-NEURO-ID) Research Project | 1 Project MembersImported from Grants Tool 4701871 Molecular Mechanisms of Neuronal Synapse Formation Research Project | 1 Project MembersImported from Grants Tool 4698088 Targeting selective mRNA translation for novel therapeutics in Autism Spectrum Disorders Research Project | 1 Project MembersImported from Grants Tool 4702406 Cellular and circuit underpinnings of social and maternal behaviours Research Project | 1 Project MembersNo Description available Development of brain-penetrant MNK inhibitors Research Project | 3 Project MembersMAP-kinase interacting kinases (MNKs) are a novel target for the treatment of autism spectrum disorders. We aim to develop a brain-penetrant, small molecule inhibitor that selectively targets MNKs and alleviates the core symptoms of autism. EMBO Fellowship for Myrto Panopoulou Research Project | 2 Project MembersNo Description available Control of molecular differentiation programs by spontaneous activity in neocortical development Research Project | 1 Project MembersThe formation of sensory cortical circuits in the mammalian brain is largely completed at the onset of sensation, with individual cortical neurons exhibiting specific and selective response properties that undergo only minor refinement thereafter. Before sensation, all sensory systems exhibit spontaneous patterned activity that propagates through ascending sensory pathways to primary cortical areas. The structure and spatio-temporal dynamics of such spontaneous patterned activity are thought to have a major impact on cortical wiring. Simultaneously, with spontaneous activity, transcriptional programs unfold that specify cortical cell types, steer their anatomical projections, and may instruct wiring specificity. Alternative mRNA splicing has emerged as a central post-transcriptional mechanism for expanding the molecular codes for neuronal wiring and synapse specification. Moreover, alterations in alternative splicing programs have been linked to neurodevelopmental disorders, in particular autism. It is unknown how spontaneous activity, transcriptional and - in particular - alternative splicing programs interact to drive neuronal wiring in cortex. In this project, we will use the mouse visual cortex as a model system to address these fundamental questions. In Aim 1, we will use in vivo two-photon calcium imaging of individual neurons to map developmental emergence of spontaneous patterned activity and will correlate spontaneous activity patterns to cell type-specific transcript isoform programs. In Aim 2, we will shift patterns of spontaneous activity in the retina and will develop novel genetic sparse marking approaches to explore the impact of patterned activity on transcript isoform programs and neuronal wiring. In Aim 3, we will uncover mechanisms underlying neuronal activity-dependent alternative splicing regulation by advancing novel genetically targeted in vivo methods for dissecting RNA-protein interactions. Together these experiments will illuminate how spontaneous activity in developing sensory systems instructs alternative splicing programs and will advance our understanding of how developmental processes mediate the acquisition of functional specificity in mature cortical networks. Finally, the results will have a profound impact on the interpretation of alternative splicing and network level defects underlying neurodevelopmental diseases. 1234 1...4 OverviewMembersPublicationsProjects & Collaborations
Projects & Collaborations 37 foundShow per page10 10 20 50 10008188 - Impact of autism spectrum disorder-associated mutations on neuronal mRNA translation Research Project | 1 Project MembersImported from Grants Tool 4721840 Molecular analysis of PBMC and brain samples from TSC patients Research Project | 2 Project MembersImported from Grants Tool 4725241 Exploring the proteomes of vulnerable synapse types in Alzheimer’s disease progression Research Project | 1 Project MembersImported from Grants Tool 4717935 Transcription Start Site determinants of Neuronal Identity (TSS-NEURO-ID) Research Project | 1 Project MembersImported from Grants Tool 4701871 Molecular Mechanisms of Neuronal Synapse Formation Research Project | 1 Project MembersImported from Grants Tool 4698088 Targeting selective mRNA translation for novel therapeutics in Autism Spectrum Disorders Research Project | 1 Project MembersImported from Grants Tool 4702406 Cellular and circuit underpinnings of social and maternal behaviours Research Project | 1 Project MembersNo Description available Development of brain-penetrant MNK inhibitors Research Project | 3 Project MembersMAP-kinase interacting kinases (MNKs) are a novel target for the treatment of autism spectrum disorders. We aim to develop a brain-penetrant, small molecule inhibitor that selectively targets MNKs and alleviates the core symptoms of autism. EMBO Fellowship for Myrto Panopoulou Research Project | 2 Project MembersNo Description available Control of molecular differentiation programs by spontaneous activity in neocortical development Research Project | 1 Project MembersThe formation of sensory cortical circuits in the mammalian brain is largely completed at the onset of sensation, with individual cortical neurons exhibiting specific and selective response properties that undergo only minor refinement thereafter. Before sensation, all sensory systems exhibit spontaneous patterned activity that propagates through ascending sensory pathways to primary cortical areas. The structure and spatio-temporal dynamics of such spontaneous patterned activity are thought to have a major impact on cortical wiring. Simultaneously, with spontaneous activity, transcriptional programs unfold that specify cortical cell types, steer their anatomical projections, and may instruct wiring specificity. Alternative mRNA splicing has emerged as a central post-transcriptional mechanism for expanding the molecular codes for neuronal wiring and synapse specification. Moreover, alterations in alternative splicing programs have been linked to neurodevelopmental disorders, in particular autism. It is unknown how spontaneous activity, transcriptional and - in particular - alternative splicing programs interact to drive neuronal wiring in cortex. In this project, we will use the mouse visual cortex as a model system to address these fundamental questions. In Aim 1, we will use in vivo two-photon calcium imaging of individual neurons to map developmental emergence of spontaneous patterned activity and will correlate spontaneous activity patterns to cell type-specific transcript isoform programs. In Aim 2, we will shift patterns of spontaneous activity in the retina and will develop novel genetic sparse marking approaches to explore the impact of patterned activity on transcript isoform programs and neuronal wiring. In Aim 3, we will uncover mechanisms underlying neuronal activity-dependent alternative splicing regulation by advancing novel genetically targeted in vivo methods for dissecting RNA-protein interactions. Together these experiments will illuminate how spontaneous activity in developing sensory systems instructs alternative splicing programs and will advance our understanding of how developmental processes mediate the acquisition of functional specificity in mature cortical networks. Finally, the results will have a profound impact on the interpretation of alternative splicing and network level defects underlying neurodevelopmental diseases. 1234 1...4
10008188 - Impact of autism spectrum disorder-associated mutations on neuronal mRNA translation Research Project | 1 Project MembersImported from Grants Tool 4721840
Molecular analysis of PBMC and brain samples from TSC patients Research Project | 2 Project MembersImported from Grants Tool 4725241
Exploring the proteomes of vulnerable synapse types in Alzheimer’s disease progression Research Project | 1 Project MembersImported from Grants Tool 4717935
Transcription Start Site determinants of Neuronal Identity (TSS-NEURO-ID) Research Project | 1 Project MembersImported from Grants Tool 4701871
Molecular Mechanisms of Neuronal Synapse Formation Research Project | 1 Project MembersImported from Grants Tool 4698088
Targeting selective mRNA translation for novel therapeutics in Autism Spectrum Disorders Research Project | 1 Project MembersImported from Grants Tool 4702406
Cellular and circuit underpinnings of social and maternal behaviours Research Project | 1 Project MembersNo Description available
Development of brain-penetrant MNK inhibitors Research Project | 3 Project MembersMAP-kinase interacting kinases (MNKs) are a novel target for the treatment of autism spectrum disorders. We aim to develop a brain-penetrant, small molecule inhibitor that selectively targets MNKs and alleviates the core symptoms of autism.
Control of molecular differentiation programs by spontaneous activity in neocortical development Research Project | 1 Project MembersThe formation of sensory cortical circuits in the mammalian brain is largely completed at the onset of sensation, with individual cortical neurons exhibiting specific and selective response properties that undergo only minor refinement thereafter. Before sensation, all sensory systems exhibit spontaneous patterned activity that propagates through ascending sensory pathways to primary cortical areas. The structure and spatio-temporal dynamics of such spontaneous patterned activity are thought to have a major impact on cortical wiring. Simultaneously, with spontaneous activity, transcriptional programs unfold that specify cortical cell types, steer their anatomical projections, and may instruct wiring specificity. Alternative mRNA splicing has emerged as a central post-transcriptional mechanism for expanding the molecular codes for neuronal wiring and synapse specification. Moreover, alterations in alternative splicing programs have been linked to neurodevelopmental disorders, in particular autism. It is unknown how spontaneous activity, transcriptional and - in particular - alternative splicing programs interact to drive neuronal wiring in cortex. In this project, we will use the mouse visual cortex as a model system to address these fundamental questions. In Aim 1, we will use in vivo two-photon calcium imaging of individual neurons to map developmental emergence of spontaneous patterned activity and will correlate spontaneous activity patterns to cell type-specific transcript isoform programs. In Aim 2, we will shift patterns of spontaneous activity in the retina and will develop novel genetic sparse marking approaches to explore the impact of patterned activity on transcript isoform programs and neuronal wiring. In Aim 3, we will uncover mechanisms underlying neuronal activity-dependent alternative splicing regulation by advancing novel genetically targeted in vivo methods for dissecting RNA-protein interactions. Together these experiments will illuminate how spontaneous activity in developing sensory systems instructs alternative splicing programs and will advance our understanding of how developmental processes mediate the acquisition of functional specificity in mature cortical networks. Finally, the results will have a profound impact on the interpretation of alternative splicing and network level defects underlying neurodevelopmental diseases.
