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Real-time viscosity sensors for label-free and functionalization-free characterization of molecular interactions

Research Project
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01.04.2013
 - 30.04.2015

This project targets the development of new bio-sensing platforms and methods for detection of molecular interactions. In contrast to other state-of-the-art bio-sensors, the proposed approach allows measuring bio-interactions without the need for labeling targeted bio-molecules or decorating the sensor surface with appropriate receptors. This research project has a considerable interest in both basic research (systems biology) and pharmaceutics (drug screening, diagnostics). We propose to use micro-viscometers (e.g. cantilevers operated in a dynamic oscillation mode) in combination with a micro-fluidic system to measure changes in (intrinsic) viscosity induced by the binding of molecules. This allows detecting molecular interactions in a label-free and a functionalization-free manner. The (intrinsic) viscosity of a solution depends on the shape, surface charge and molecular weight of the solved molecules. If two molecules (say A and B) bind to each other to form a new complex (having a combined molecular weight and shape), the intrinsic viscosity changes. Since the intrinsic viscosity is an additive quantity, comparing the intrinsic viscosities of A, B and A+B allows the detection of the interaction between A and B.

Members (2)

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Thomas Braun

Principal Investigator
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Christoph Gerber

Co-Investigator