Prof. Dr. Markus Affolter

Belting, Heinz-Georg, Maggi, Ludovico, Yin, Jianmin, Paatero, Ilkka, Wiesner, Cora, Malchow, Julian, Helker, Christian, & Affolter Markus. (2025). Junctional and Actomyosin Dynamics Drive Endothelial Cell Rearrangements During Vascular Tube Formation. In Research Square (18.07.2025). Research Square. https://doi.org/10.21203/rs.3.rs-7065344/v1

Jiménez-Jiménez, Carlos, Aguilar, Gustavo, Fernandez-Pardo, Clara, Affolter, Markus, & Guerrero, Isabel. (2025). Direct cell-to-cell transport of Hedgehog morphogen is aided by the diffusible carrier Shifted/DmWif1. In bioRxiv. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2025.06.04.657875

Bao, Mengjing, Lepeta, Katarzyna, Aguilar, Gustavo, Schnider, Sophie, Affolter, Markus, & Vigano, Maria Alessandra. (2025). In vivo regulation of an endogenously-tagged protein by a light-regulated kinase [Journal-article]. G3: Genes, Genomes, Genetics, 15(6). https://doi.org/10.1093/g3journal/jkaf073

Reinger, Cindy, Blackie, Laura, Gillet, Hugo, Gaspar, Pedro, Medeiros, Alexandra M., Hadjieconomou, Dafni, Sickmann, Michèle, Affolter, Markus, Miguel-Aliaga, Irene, Kempf, Anissa, & Müller, Martin. (2025). Intestinal control of feeding initiation in Drosophila melanogaster. In 10.1101/2025.03.31.646269. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2025.03.31.646269

Bao, Mengjing, Lepeta, Katarzyna, Aguilar, Gustavo, Schnider, Sophie, Affolter, Markus, & Vigano, Maria Alessandra. (2024). In vivo regulation of an endogenously-tagged protein by a light-regulated kinase [Posted-content]. In bioRxiv. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2024.11.27.625702

Yin, Jianmin, Schellinx, Niels, Maggi, Ludovico, Gundel, Kathrin, Wiesner, Cora, Kotini, Maria Paraskevi, Lee, Minkyoung, Phng, Li-Kun, Belting, Heinz-Georg, & Affolter, Markus. (2024). Initiation of lumen formation from junctions via differential actomyosin contractility regulated by dynamic recruitment of Rasip1 [Journal-article]. Nature Communications, 15(1). https://doi.org/10.1038/s41467-024-54143-y

Yin, Jianmin, Maggi, Ludovico, Wiesner, Cora, Affolter, Markus, & Belting, Heinz-Georg. (2024). Oscillatory contractile forces refine endothelial cell-cell interactions for continuous lumen formation governed by Heg1/Ccm1 [Journal-article]. Angiogenesis, 27(4), 845–860. https://doi.org/10.1007/s10456-024-09945-5

Aguilar, Gustavo, Bauer, Milena, Vigano, M. Alessandra, Guerrero, Isabel, & Affolter, Markus. (2024). Protocol for generating in-frame seamless knockins in Drosophila using the SEED/Harvest technology [Journal-article]. STAR Protocols, 5(3). https://doi.org/10.1016/j.xpro.2024.102932

Schnider, Sophie T., Vigano, M. Alessandra, Affolter, Markus, & Aguilar, Gustavo. (2024). Functionalized Protein Binders in Developmental Biology [Journal-article]. Annual Review of Cell and Developmental Biology, 40(1), 119–142. https://doi.org/10.1146/annurev-cellbio-112122-025214

Aguilar, Gustavo, Bauer, Milena, Vigano, M Alessandra, Schnider, Sophie T, Brugger, Lukas, Jiménez-Jiménez, Carlos, Guerrero, Isabel, & Affolter, Markus. (2024). Seamless knockins in Drosophila via CRISPR-triggered single-strand annealing. Developmental Cell, October 2024, Online ahead of print. https://doi.org/10.1016/j.devcel.2024.06.004

Aguilar, Gustavo, Aguilar, Gustavo, Sickmann, Michèle, Bieli, Dimitri, Born, Gordian, Affolter, Markus, & Müller, Martin. (2023). Transcriptional control of compartmental boundary positioning during Drosophila wing development. eLife. https://doi.org/10.7554/elife.91713.1

Aguilar, Gustavo, Sickmann, Michèle, Born, Gordian, Bieli, Dimitri, Affolter, Markus, & Müller, Müller. (2023). Transcriptional control of compartmental boundary positioning during Drosophila wing development. bioRxiv Cold Spring Harbor Laboratory. https://doi.org/10.1101/2023.08.05.552106

Bauer, Milena, Aguilar, Gustavo, Wharton, Kristi A., Matsuda, Shinya, & Affolter, Markus. (2023). Heterodimerization-dependent secretion of bone morphogenetic proteins in Drosophila. Developmental Cell, Online Ahead of Print, Epub. https://doi.org/10.1016/j.devcel.2023.03.008

Born, Gordian, Bieli, Dimi, Metzler, Mario, Gohl, Daryl M., Affolter, Markus, & Müller, Martin. (2023). No apparent role for the Wari insulator in transcriptional regulation of the endogenous white gene of Drosophila melanogaster. microPublication Biology, 2023, 702. https://doi.org/10.17912/micropub.biology.000702

Heutschi, Daniel, Schmelzer, Etienne, Aydogan, Vahap, Schmidt, Alexander, Belting, Heinz-Georg, Spang, Anne, Affolter, Markus, & Kotini, Maria P. (2023). Genetic analysis of rab7 mutants in zebrafish. bioRxiv. https://doi.org/10.1101/2023.03.09.531857

Kemmler, Cassie L., Moran, Hannah R., Murray, Brooke F., Scoresby, Aaron, Klem, John R., Eckert, Rachel L., Lepovsky, Elizabeth, Bertho, Sylvain, Nieuwenhuize, Susan, Burger, Sibylle, D’Agati, Gianluca, Betz, Charles, Puller, Ann-Christin, Felker, Anastasia, Ditrychova, Karolina, Bötschi, Seraina, Affolter, Markus, Rohner, Nicolas, Lovely, C. Ben, et al. (2023). Next-generation plasmids for transgenesis in zebrafish and beyond. Development, 150(8), 1–54. https://doi.org/10.1242/dev.201531

Matsuda, Shinya, & Affolter, Markus. (2023). Is Drosophila Dpp/BMP morphogen spreading required for wing patterning and growth? BioEssays : news and reviews in molecular, cellular and developmental biology, e2200218. https://doi.org/10.1002/bies.202200218

Aguilar, Gustavo, Bauer, Milena, Vigano, M. Alessandra, Schnider, Sophie T., Brügger, Lukas, Jiménez-Jiménez, Carlos, Guerrero, Isabel, & Affolter, Markus. (2022). In vivo seamless genetic engineering via CRISPR-triggered single-strand annealing [Posted-content]. In bioRxiv. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2022.06.17.496589

Aguilar, Gustavo, Bauer, Milena, Vigano, M. Alessandra, Jiménez-Jiménez, Carlos, Guerrero, Isabel, & Affolter, Markus. (2022). In vivo seamless genetic engineering via CRISPR-triggered single-strand annealing. bioRxiv. https://doi.org/10.1101/2022.06.17.496589v1

Bauer, Milena, Aguilar, Gustavo, Wharton, Kristi A., Matsuda, Shinya, & Affolter, Markus. (2022). Heterodimerization-dependent secretion of BMPs in Drosophila. bioRxiv. https://doi.org/10.1101/2022.08.04.502599

Kemmler Cassie L: Moran, MoHannah R., F. Murray, Brooke, Scoresby, Aaron, Klem, John R., Eckert, Rachel L., Lepovsky, Elizabeth, Bertho, Sylvain, Nieuwenhuize, Susan, Burger, Sibylle, D’Agati, Gianluca, Betz, Charles, Puller, Ann-Christin, Felker, Anastasia, Ditrychová, Karolina, Bötschi, Seraina, Affolter, Markus, Rohner, Nicolas, Lovely, C. Ben, Kwan M, Kristen, et al. (2022). Next-generation plasmids for transgenesis in zebrafish and beyond. bioRxiv. https://doi.org/10.1101/2022.12.13.520107

Kotini, Maria P., van der Stoel, Miesje M., Yin, Jianmin, Han, Mitchell K., Kirchmaier, Bettina, de Rooij, Johan, Affolter, Markus, Huveneers, Stephan, & Belting, Heinz-Georg. (2022). Vinculin controls endothelial cell junction dynamics during vascular lumen formation. Cell reports, 39(2), 110658. https://doi.org/10.1016/j.celrep.2022.110658

Lepeta, Katarzyna, Bauer, Milena, Aguilar, Gustavo, Vigano, M. Alessandra, Matsuda, Shinya, & Affolter, Markus. (2022). Studying Protein Function Using Nanobodies and Other Protein Binders in Drosophila. Methods in Molecular Biology, 2540, 219–237. https://doi.org/10.1007/978-1-0716-2541-5_10

Lepeta, Katarzyna, Roubinet, Chantal, Bauer, Milena, Vigano, M. Alessandra, Aguilar, Gustavo, Kanca, Oguz, Ochoa-Espinosa, Amanda, Bieli, Dimitri, Cabernard, Clemens, Caussinus, Emmanuel, & Affolter, Markus. (2022). Engineered kinases as a tool for phosphorylation of selected targets in vivo. Journal of Cell Biology, 221(10), e202106179. https://doi.org/10.1083/jcb.202106179

Matsuda, Shinya, Aguilar, Gustavo, Vigano, M. Alessandra, & Affolter, Markus. (2022). Nanobody-Based GFP Traps to Study Protein Localization and Function in Developmental Biology. Methods in Molecular Biology, 2446, 581–593. https://doi.org/10.1007/978-1-0716-2075-5_30

Matsuda, Shinya, Schaefer, Jonas V., Mii, Yusuke, Hori, Yutaro, Bieli, Dimitri, Taira, Masanori, Plückthun, Andreas, & Affolter, Markus. (2022). Author Correction: Asymmetric requirement of Dpp/BMP morphogen dispersal in the Drosophila wing disc. Nature Communications, 13(1), 389. https://doi.org/10.1038/s41467-021-27680-z

Mesrouze, Yannick, Aguilar, Gustavo, Meyerhofer, Marco, Bokhovchuk, Fedir, Zimmermann, Catherine, Fontana, Patrizia, Vissières, Alexandra, Voshol, Hans, Erdmann, Dirk, Affolter, Markus, & Chène, Patrick. (2022). The role of lysine palmitoylation/myristoylation in the function of the TEAD transcription factors. Scientific Reports, 12(1), 4984. https://doi.org/10.1038/s41598-022-09127-7

van der Stoel, Miesje M., Kotini, Maria P., Schoon, Rianne M., Affolter, Markus, Belting, Heinz-Georg, & Huveneers, Stephan. (2022). Vinculin strengthens the endothelial barrier during vascular development. Vascular Biology, 5(1), e220012. https://doi.org/10.1530/vb-22-0012

Vigano, M. Alessandra, Ell, Clara-Maria, Kustermann, Manuela M. M., Aguilar, Gustavo, Matsuda, Shinya, Zhao, Ning, Stasevich, Timothy J., Affolter, Markus, & Pyrowolakis, George. (2021). Protein manipulation using single copies of short peptide tags in cultured cells and in; Drosophila melanogaster; Development, 148(6). https://doi.org/10.1242/dev.191700

Affolter, Markus. (2021). Preface. Current Topics in Developmental Biology, 143, xi–xiv. https://doi.org/10.1016/s0070-2153(21)00040-5

Kotini, Maria P., Bachmann, Felix, Spickermann, Jochen, McSheehy, Paul M., & Affolter, Markus. (2021). Probing the Effects of the FGFR-Inhibitor Derazantinib on Vascular Development in Zebrafish Embryos. Pharmaceuticals, 14(1), 25. https://doi.org/10.3390/ph14010025

Lee, Minkyoung, Betz, Charles, Yin, Jianmin, Paatero, Ilkka, Schellinx, Niels, Carte, Adam N., Wilson, Christopher W., Ye, Weilan, Affolter, Markus, & Belting, Heinz-Georg. (2021). Control of dynamic cell behaviors during angiogenesis and anastomosis by Rasip1. Development, 148(15), dev197509. https://doi.org/10.1242/dev.197509

Matsuda, Shinya, Schaefer, Jonas V., Mii, Yusuke, Hori, Yutaro, Bieli, Dimitri, Taira, Masanori, Plückthun, Andreas, & Affolter, Markus. (2021). Asymmetric requirement of Dpp/BMP morphogen dispersal in the Drosophila wing disc. Nature Communications, 12(1), 6435. https://doi.org/10.1038/s41467-021-26726-6

Yang, Zhenguo, Wu, Shuilong, Fontana, Federica, Li, Yanyu, Xiao, Wei, Gao, Zhangdai, Krudewig, Alice, Affolter, Markus, Belting, Heinz-Georg, Abdelilah-Seyfried, Salim, & Zhang, Jingjing. (2021). The tight junctions protein Claudin-5 limits endothelial cell motility. Journal of Cell Science, 134(1), jcs248237. https://doi.org/10.1242/jcs.248237

Yin, Jianmin, Heutschi, Daniel, Belting, Heinz-Georg, & Affolter, Markus. (2021). Building the complex architectures of vascular networks: Where to branch, where to connect and where to remodel? Current Topics in Developmental Biology, 143, 281–297. https://doi.org/10.1016/bs.ctdb.2021.01.002

Vigano, M. Alessandra, Ell, Clara-Maria, Kustermann, Manuela MM, Aguilar, Gustavo, Matsuda, Shinya, Zhao, Ning, Stasevich, Timothy J, Pyrowolakis, George, & Affolter, Markus. (2020). Protein manipulation using single copies of short peptide tags in cultured cells and in Drosophila melanogaster [Posted-content]. In bioRxiv. Cold Spring Harbor Laboratory. https://doi.org/10.1101/2020.04.06.027599

Galeone, Antonio, Adams, Joshua M., Matsuda, Shinya, Presa, Maximiliano F., Pandey, Ashutosh, Han, Seung Yeop, Tachida, Yuriko, Hirayama, Hiroto, Vaccari, Thomas, Suzuki, Tadashi, Lutz, Cathleen M., Affolter, Markus, Zuberi, Aamir, & Jafar-Nejad, Hamed. (2020). Regulation of BMP4/Dpp retrotranslocation and signaling by deglycosylation. eLife, 9, e55596. https://doi.org/10.7554/elife.55596

Mesrouze, Yannick, Aguilar, Gustavo, Bokhovchuk, Fedir, Martin, Typhaine, Delaunay, Clara, Villard, Frédéric, Meyerhofer, Marco, Zimmermann, Catherine, Fontana, Patrizia, Wille, Roman, Vorherr, Thomas, Erdmann, Dirk, Furet, Pascal, Scheufler, Clemens, Schmelzle, Tobias, Affolter, Markus, & Chène, Patric. (2020). A new perspective on the evolution of the interaction between the Vg/VGLL1-3 proteins and the TEAD transcription factors. Scientific Reports, 10, 17442. https://doi.org/10.1038/s41598-020-74584-x

Aguilar, Gustavo, Matsuda, Shinya, Vigano, M. Alessandra, & Affolter, Markus. (2019). Using Nanobodies to Study Protein Function in Developing Organisms. Antibodies, 8(1), 16. https://doi.org/10.3390/antib8010016

Aguilar, Gustavo, Vigano, M. Alessandra, Affolter, Markus, & Matsuda, Shinya. (2019). Reflections on the use of protein binders to study protein function in developmental biology. WIREs Developmental Biology, 8(6), e356. https://doi.org/10.1002/wdev.356

Angulo-Urarte, Ana, Casado, Pedro, Castillo, Sandra D., Kobialka, Piotr, Kotini, Maria Paraskevi, Figueiredo, Ana M., Castel, Pau, Rajeeve, Vinothini, Milà-Guasch, Maria, Millan, Jaime, Wiesner, Cora, Serra, Helena, Muixi, Laia, Casanovas, Oriol, Viñals, Francesc, Affolter, Markus, Gerhardt, Holger, Huveneers, Stephan, Belting, Heinz-Georg, et al. (2018). Endothelial cell rearrangements during vascular patterning require PI3-kinase-mediated inhibition of actomyosin contractility. Nature Communications, 9(1), 4826. https://doi.org/10.1038/s41467-018-07172-3

Harmansa, Stefan, & Affolter, Markus. (2018). Protein binders and their applications in developmental biology. Development, 145(2), 1–13. https://doi.org/10.1242/dev.148874

Hübner, Kathleen, Cabochette, Pauline, Diéguez-Hurtado, Rodrigo, Wiesner, Cora, Wakayama, Yuki, Grassme, Kathrin S., Hubert, Marvin, Guenther, Stefan, Belting, Heinz-Georg, Affolter, Markus, Adams, Ralf H., Vanhollebeke, Benoit, & Herzog, Wiebke. (2018). Wnt/β-catenin signaling regulates VE-cadherin-mediated anastomosis of brain capillaries by counteracting S1pr1 signaling. Nature Communications, 9(1), 4860. https://doi.org/10.1038/s41467-018-07302-x

Kotini, Maria Paraskevi, Mäe, Maarja Andaloussi, Belting, Heinz-Georg, Betsholtz, Christer, & Affolter, Markus. (2018). Sprouting and anastomosis in the Drosophila trachea and the vertebrate vasculature: Similarities and differences in cell behaviour. Vascular Pharmacology, 112, 8–16. https://doi.org/10.1016/j.vph.2018.11.002

Paatero, Ilkka, Sauteur, Loïc, Lee, Minkyoung, Lagendijk, Anne K., Heutschi, Daniel, Wiesner, Cora, Guzmán, Camilo, Bieli, Dimitri, Hogan, Benjamin M., Affolter, Markus, & Belting, Heinz-Georg. (2018). Junction-based lamellipodia drive endothelial cell rearrangements in vivo via a VE-cadherin-F-actin based oscillatory cell-cell interaction. Nature Comm, 9(1), 3545. https://doi.org/10.1038/s41467-018-05851-9

Postika, Nikolay, Metzler, Mario, Affolter, Markus, Müller, Martin, Schedl, Paul, Georgiev, Pavel, & Kyrchanova, Olga. (2018). Boundaries mediate long-distance interactions between enhancers and promoters in the Drosophila Bithorax complex. PLoS Genet, 14(12), e1007702. https://doi.org/10.1371/journal.pgen.1007702

Sickmann, Michèle, Affolter, Markus, & Müller, Martin. (2018). Characterization of the new bithorax allele Ubx bx-Basel. Drosophila Information Service, 101, 76–80. http://www.ou.edu/journals/dis/DIS101/DIS101.html

Vigano, M. Alessandra, Bieli, Dimitri, Schaefer, Jonas V., Jakob, Roman Peter, Matsuda, Shinya, Maier, Timm, Plückthun, Andreas, & Affolter, Markus. (2018). DARPins recognizing mTFP1 as novel reagents for in vitro and in vivo protein manipulations. Biology Open, 7(11), bio036749. https://doi.org/10.1242/bio.036749

Vigano, M. Alessandra, Bieli, Dimitri, Schaefer, Jonas V., Jakob, Roman P., Matsuda, Shinya, Maier, Timm, Plückthun, Andreas, & Affolter, Markus. (2018). Correction:DARPins recognizing mTFP1 as novel reagents for in vitro; and in vivo protein manipulations. Biology Open, 7(12), bio036749. https://doi.org/10.1242/bio.040832

Affolter, Markus. (2017). Fluoreszierende Eiweisse: von der Natur ins Labor. In Füglister, Kurt M.; Hicklin, Martin; Mäser, Pascal (Ed.), natura obscura. 200 Naturforschende - 200 Naturphänomene - 200 Jahre Naturforschende Gesellschaft in Basel (pp. 9). Schwabe.

Harmansa, Stefan, Alborelli, Ilaria, Bieli, Dimitri, Caussinus, Emmanuel, & Affolter, Markus. (2017). A nanobody-based toolset to investigate the role of protein localization and dispersal in Drosophila. eLife, 6, 22549. https://doi.org/10.7554/elife.22549

Lagendijk, Anne Karine, Gomez, Guillermo A., Baek, Sungmin, Hesselson, Daniel, Hughes, William E., Paterson, Scott, Conway, Daniel E., Belting, Heinz-Georg, Affolter, Markus, Smith, Kelly A., Schwartz, Martin A., Yap, Alpha S., & Hogan, Benjamin M. (2017). Live imaging molecular changes in junctional tension upon VE-cadherin in zebrafish. Nature Communications, 8(1), 1402. https://doi.org/10.1038/s41467-017-01325-6

Matsuda, Shinya, & Affolter, Markus. (2017). Dpp from the anterior stripe of cells is crucial for the growth of the Drosophila wing disc. eLife, 6, e22319. https://doi.org/10.7554/elife.22319

Nakajima, Hiroyuki, Yamamoto, Kimiko, Agarwala, Sobhika, Terai, Kenta, Fukui, Hajime, Fukuhara, Shigetomo, Ando, Koji, Miyazaki, Takahiro, Yokota, Yasuhiro, Schmelzer, Etienne, Belting, Heinz-Georg, Affolter, Markus, Lecaudey, Virginie, & Mochizuki, Naoki. (2017). Flow-Dependent Endothelial YAP Regulation Contributes to Vessel Maintenance. Developmental Cell, 40(6), 523–536. https://doi.org/10.1016/j.devcel.2017.02.019

Ochoa-Espinosa, Amanda, Harmansa, Stefan, Caussinus, Emmanuel, & Affolter, Markus. (2017). Myosin II is not required for Drosophila tracheal branch elongation and cell intercalation. Development, 144(16), 2961–2968. https://doi.org/10.1242/dev.148940

Paatero, Ilkka, Sauteur, Loïc, Lee, Minkyoung, Lagendijk, Anne K., Heutschi, Daniel, Bieli, Dimitri, Hogan, Benjamin M., Affolter, Markus, & Belting, Heinz-Georg. (2017). Junction-based lamellipodia drive endothelial cell arrangements in vivo via a VE-cadherin/F-actin based oscillatory ratchet mechanism. bioRxiv, 1–32. https://doi.org/10.1101/212522

Roubinet, Chantal, Tsankova, Anna, Pham, Tri Thanh, Monnard, Arnaud, Caussinus, Emmanuel, Affolter, Markus, & Cabernard, Clemens. (2017). Spatio-temporally separated cortical flows and spindle geometry establish physical asymmetry in fly neural stem cells. Nature Communications, 8(1), 1383. https://doi.org/10.1038/s41467-017-01391-w

Sauteur, Loïc, Affolter, Markus, & Belting, Heinz-Georg. (2017). Distinct and redundant functions of Esam and VE-cadherin during vascular morphogenesis. Development, 144(8), 1554–1565. https://doi.org/10.1242/dev.140038

Sickmann, Michèle, Gohl, Daryl M., Affolter, Markus, & Müller, Martin. (2017). The Drosophila melanogaster straw locus is allelic to laccase2. Drosophila Information Service, 100, 171–174. http://www.ou.edu/journals/dis/DIS100/DIS%20100.pdf

Tsachaki, Maria, Meyer, Arne, Weger, Benjamin, Kratschmar, Denise V., Tokarz, Janina, Adamski, Jerzy, Belting, Heinz-Georg, Affolter, Markus, Dickmeis, Thomas, & Odermatt, Alex. (2017). Absence of 11-keto reduction of cortisone and 11-ketotestosterone in the model organism zebrafish. Journal of Endocrinology, 232(2), 323–335. https://doi.org/10.1530/joe-16-0495

Affolter, Markus. (2016). Seeing is Believing, or How GFP Changed My Approach to Science. Current Topics in Developmental Biology, 116, 1–16. https://doi.org/10.1016/bs.ctdb.2015.12.001

Aydogan, Vahap, Belting, Heinz-Georg, & Affolter, Markus. (2016). Mapping the molecular steps of secretory-lysosome-driven tracheal tube fusion. Nature Cell Biology, 18(7), 2–720. https://doi.org/10.1038/ncb3383

Betz, Charles, Lenard, Anna, Belting, Heinz-Georg, & Affolter, Markus. (2016). Cell behaviors and dynamics during angiogenesis. Development, 143(13), 60–2249. https://doi.org/10.1242/dev.135616

Bieli, D., Alborelli, I., Harmansa, S., Matsuda, S., Caussinus, E., & Affolter, Markus. (2016). Development and Application of Functionalized Protein Binders in Multicellular Organisms. International Review of Cell and Molecular Biology, 325, 181–213. https://doi.org/10.1016/bs.ircmb.2016.02.006

Caussinus, Emmanuel, & Affolter, Markus. (2016). deGradFP: A System to Knockdown GFP-Tagged Proteins. Methods in Molecular Biology, 1478, 177–187. https://doi.org/10.1007/978-1-4939-6371-3_9

Matsuda, Shinya, Harmansa, Stefan, & Affolter, Markus. (2016). BMP morphogen gradients in flies. Cytokine & Growth Factor Reviews, 27, 27–119. https://doi.org/10.1016/j.cytogfr.2015.11.003

Pasakarnis, Laurynas, Frei, Erich, Caussinus, Emmanuel, Affolter, Markus, & Brunner, Damian. (2016). Amnioserosa cell constriction but not epidermal actin cable tension autonomously drives dorsal closure. Nature Cell Biology, 18(11), 1161–1172. https://doi.org/10.1038/ncb3420

Affolter, Markus. (2015). Nachruf auf Walter J. Gehring. Zoologie: Mitteilungen Der Deutschen Zoologischen Gesellschaft, 2015, 73–80.

Affolter, Markus, & Nigg, Erich A. (2015). In memoriam - Walter J. Gehring. Chromosoma, 124(3), 291. https://doi.org/10.1007/s00412-015-0523-z

Aydogan, Vahap, Lenard, Anna, Denes, Alexandru Stefan, Sauteur, Loic, Belting, Heinz-Georg, & Affolter, Markus. (2015). Endothelial cell division in angiogenic sprouts of differing cellular architecture. Biology open, 4(10), 69–1259. https://doi.org/10.1242/bio.012740

Bieli, Dimitri, Kanca, Oguz, Gohl, Daryl, Denes, Alexandru, Schedl, Paul, Affolter, Markus, & Müller, Martin. (2015). The drosophila melanogaster mutants apblot and apXasta affect an essential apterous wing enhancer. G3 : Genes, Genomes, Genetics, 5(6), 1129–1143. https://doi.org/10.1534/g3.115.017707

Bieli, Dimitri, Kanca, Oguz, Requena, David, Hamaratoglu, Fisun, Gohl, Daryl, Schedl, Paul, Affolter, Markus, Slattery, Matthew, Müller, Martin, & Estella, Carlos. (2015). Establishment of a Developmental Compartment Requires Interactions between Three Synergistic Cis-regulatory Modules. PLoS Genetics, 11(10), e1005376. https://doi.org/10.1371/journal.pgen.1005376

Bürglin, Thomas R., & Affolter, Markus. (2015). Homeodomain proteins: an update. Chromosoma, 125(3), 497–521. https://doi.org/10.1007/s00412-015-0543-8

Denes, Alexandru Stefan, Kanca, Oguz, & Affolter, Markus. (2015). A cellular process that includes asymmetric cytokinesis remodels the dorsal tracheal branches in Drosophila larvae. Development, 142(10), 1794–1805. https://doi.org/10.1242/dev.118372

Harmansa, Stefan, Hamaratoglu, Fisun, Affolter, Markus, & Caussinus, Emmanuel. (2015). Dpp spreading is required for medial but not for lateral wing disc growth. Nature, 527(7578), 22–317. https://doi.org/10.1038/nature15712

Ittig, Simon J., Schmutz, Christoph, Kasper, Christoph A., Amstutz, Marlise, Schmidt, Alexander, Sauteur, Loic, Vigano, M. Alessandra, Low, Shyan Huey, Affolter, Markus, Cornelis, Guy R., Nigg, Erich A., & Arrieumerlou, Cecile. (2015). A bacterial type III secretion-based protein delivery tool for broad applications in cell biology. The Journal of Cell Biology, 211(4), 913–931. https://doi.org/10.1083/jcb.201502074

Lenard, Anna, Daetwyler, Stephan, Betz, Charles, Ellertsdottir, Elin, Belting, Heinz-Georg, Huisken, Jan, & Affolter, Markus. (2015). Endothelial Cell Self-fusion during Vascular Pruning. PLoS Biology, 13(4), e1002126. https://doi.org/10.1371/journal.pbio.1002126

Phng, Li-Kun, Gebala, Véronique, Bentley, Katie, Philippides, Andrew, Wacker, Andrin, Mathivet, Thomas, Sauteur, Loïc, Stanchi, Fabio, Belting, Heinz-Georg, Affolter, Markus, & Gerhardt, Holger. (2015). Formin-mediated actin polymerization at endothelial junctions is required for vessel lumen formation and stabilization. Developmental Cell, 32(1), 32–123. https://doi.org/10.1016/j.devcel.2014.11.017

Affolter, Markus, & Müller, Martin. (2014). Walter Jakob Gehring (1939-2014). Developmental Cell, 30(2), 2–120. https://doi.org/10.1016/j.devcel.2014.07.011

Affolter, Markus, & Wüthrich, Kurt. (2014). Walter Jakob Gehring: a Master of Developmental Biology. Proceedings of the National Academy of Sciences of the United States of America, 111(35), 5–12574. https://doi.org/10.1073/pnas.1413434111

Brauchle, Michael, Hansen, Simon, Caussinus, Emmanuel, Lenard, Anna, Ochoa-Espinosa, Amanda, Scholz, Oliver, Sprecher, Simon G, Plückthun, Andreas, & Affolter, Markus. (2014). Protein interference applications in cellular and developmental biology using DARPins that recognize GFP and mCherry. Biology Open, 3(12), 61–1252. https://doi.org/10.1242/bio.201410041

De Smet, Frederik, Tembuyser, Bieke, Lenard, Anna, Claes, Filip, Zhang, Jie, Michielsen, Christof, Van Schepdael, Ann, Herbert, Jean-Marc, Bono, Françoise, Affolter, Markus, Dewerchin, Mieke, & Carmeliet, Peter. (2014). Fibroblast Growth Factor Signaling Affects Vascular Outgrowth and Is Required for the Maintenance of Blood Vessel Integrity. Chemistry & Biology, 21(10), 1310–1317. https://doi.org/10.1016/j.chembiol.2014.07.018

Hultin, Sara, Zheng, Yujuan, Mojallal, Mahdi, Vertuani, Simona, Gentili, Christian, Balland, Martial, Milloud, Rachel, Belting, Heinz-Georg, Affolter, Markus, Helker, Christian S M, Adams, Ralf H, Herzog, Wiebke, Uhlen, Per, Majumdar, Arindam, & Holmgren, Lars. (2014). AmotL2 Links VE-cadherin to Contractile Actin Fibres Necessary for Aortic Lumen Expansion. Nature Communications, 5, 3743. https://doi.org/10.1038/ncomms4743

Kanca, Oguz, Caussinus, Emmanuel, Denes, Alexandru S, Percival-Smith, Anthony, & Affolter, Markus. (2014). Raeppli: a whole-tissue labeling tool for live imaging of Drosophila development. Development, 141(2), 80–472. https://doi.org/10.1242/dev.102913

Kanca, Oguz, Ochoa-Espinosa, Amanda, & Affolter, Markus. (2014). IV. Tools and Methods for Studying Cell Migration and Cell Rearrangement in Tissue and Organ Development. Methods, 68(1), 32–228. https://doi.org/10.1016/j.ymeth.2014.03.004

Santos, M. Emília, Braasch, Ingo, Boileau, Nicolas, Meyer, Britta S., Sauteur, Loïc, Böhne, Astrid, Belting, Heinz-Georg, Affolter, Markus, & Salzburger, Walter. (2014). The evolution of cichlid fish egg-spots is linked with a cis-regulatory change. Nature Communications, 5, 5149. https://doi.org/10.1038/ncomms6149

Sauteur, Loïc, Krudewig, Alice, Herwig, Lukas, Ehrenfeuchter, Nikolaus, Lenard, Anna, Affolter, Markus, & Belting, Heinz-Georg. (2014). Cdh5/VE-cadherin Promotes Endothelial Cell Interface Elongation via Cortical Actin Polymerization during Angiogenic Sprouting. Cell Reports, 9(2), 13–504. https://doi.org/10.1016/j.celrep.2014.09.024

Caussinus, Emmanuel, Kanca, Oguz, & Affolter, Markus. (2013). Protein knockouts in living eukaryotes using deGradFP and green fluorescent protein fusion targets. Current Protocols in Protein Science, 73(30.2), Unit 30.2. https://doi.org/10.1002/0471140864.ps3002s73

Hayashi, Makoto, Majumdar, Arindam, Li, Xiujuan, Adler, Jeremy, Sun, Zuyue, Vertuani, Simona, Hellberg, Carina, Mellberg, Sofie, Koch, Sina, Dimberg, Anna, Koh, Gou Young, Dejana, Elisabetta, Belting, Heinz-Georg, Affolter, Markus, Thurston, Gavin, Holmgren, Lars, Vestweber, Dietmar, & Claesson-Welsh, Lena. (2013). VE-PTP regulates VEGFR2 activity in stalk cells to establish endothelial cell polarity and lumen formation. Nature Communications, 4, 1672. https://doi.org/10.1038/ncomms2683

Helker, Christian S M, Schuermann, Annika, Karpanen, Terhi, Zeuschner, Dagmar, Belting, Heinz-Georg, Affolter, Markus, Schulte-Merker, Stefan, & Herzog, Wiebke. (2013). The Zebrafish Common Cardinal Veins Develop by a Novel Mechanism: Lumen Ensheathment. Development, 140(13), 86–2776. https://doi.org/10.1242/dev.091876

Kochhan, Eva, Lenard, Anna, Ellertsdottir, Elin, Herwig, Lukas, Affolter, Markus, Belting, Heinz-Georg, & Siekmann, Arndt F. (2013). Blood Flow Changes Coincide with Cellular Rearrangements during Blood Vessel Pruning in Zebrafish Embryos. PLoS ONE, 8(10), e75060. https://doi.org/10.1371/journal.pone.0075060

Lenard, Anna, & Affolter, Markus. (2013). Tugging organs into place. Developmental Cell, 27(3), 243–244. https://doi.org/10.1016/j.devcel.2013.10.021

Lenard, Anna, Ellertsdottir, Elin, Herwig, Lukas, Krudewig, Alice, Sauteur, Loïc, Belting, Heinz-Georg, & Affolter, Markus. (2013). In Vivo Analysis Reveals a Highly Stereotypic Morphogenetic Pathway of Vascular Anastomosis. Developmental Cell, 25(5), 492–506. https://doi.org/10.1016/j.devcel.2013.05.010

Siekmann, Arndt F., Affolter, Markus, & Belting, Heinz-Georg. (2013). The Tip Cell Concept Ten Years After : New Players Tune in for a Common Theme. Experimental Cell Research, 319(9), 1255–1263. https://doi.org/10.1016/j.yexcr.2013.01.019

Ochoa-Espinosa, Amanda, & Affolter, Markus. (2012). Branching Morphogenesis: From Cells to Organs and Back. In Mammalian Development: Networks, Switches, and Morphogenetic Processes (pp. 81–94). Cold Spring Harbor Laboratory Press. https://doi.org/10.1101/cshperspect.a008243

Baer, Magdalena M., Palm, Wilhelm, Eaton, Suzanne, Leptin, Maria, & Affolter, Markus. (2012). Microsomal triacylglycerol transfer protein (MTP) is required cell autonomously to expand tracheal lumen in Drosophila. Journal of Cell Science, 125(24), 6038–6048. https://doi.org/10.1242/jcs.110452

Caussinus, Emmanuel, Kanca, Oguz, & Affolter, Markus. (2012). Fluorescent fusion protein knockout mediated by anti-GFP nanobody. Nature Structural & Molecular Biology, 19(1), 21–117. https://doi.org/10.1038/nsmb.2180

Ellertsdottir, Elin, Berthold, Peter R., Bouzaffour, Mohamed, Dufourcq, Pascale, Trayer, Vincent, Gauron, Carole, Vriz, Sophie, Affolter, Markus, & Rampon, Christine. (2012). Developmental Role of Zebrafish Protease-Activated Receptor 1 (PAR1) in the Cardio-Vascular System. PLoS ONE, 7(7), e42131. https://doi.org/10.1371/journal.pone.0042131

Ochoa-Espinosa, Amanda, & Affolter, Markus. (2012). Branching morphogenesis : from cells to organs and back. Cold Spring Harbor Perspectives in Biology, 4(10), 1–15. https://doi.org/10.1101/cshperspect.a008243

Ochoa-Espinosa, Amanda, Baer, Magdalena M, & Affolter, Markus. (2012). Tubulogenesis: Src42A Goes to Great Lengths in Tube Elongation. Current Biology, 22(11), R446–9. https://doi.org/10.1016/j.cub.2012.04.033