Prof. Dr. Florian Peter Seebeck

Nalivaiko, Egor Y., & Seebeck, Florian P. (2024). A Rhodanese‐Like Enzyme that Catalyzes Desulfination of Ergothioneine Sulfinic Acid. ChemBioChem, 25(9). https://doi.org/10.1002/cbic.202400131

Vasseur, Camille M., Karunasegaram, Dishani, & Seebeck, Florian P. (2024). Structure and Substrate Specificity of S -Methyl Thiourocanate Hydratase. ACS Chemical Biology, 19(3), 718–724. https://doi.org/10.1021/acschembio.3c00745

Nalivaiko, Egor Y., Vasseur, Camille M., & Seebeck, Florian P. (2024). Enzyme‐Catalyzed Oxidative Degradation of Ergothioneine. Angewandte Chemie International Edition, 63(8). https://doi.org/10.1002/anie.202318445

Peng, Jiaming, Hughes, Gregory R., Müller, Manuel M., & Seebeck, Florian P. (2024). Enzymatic Fluoromethylation as a Tool for ATP‐Independent Ligation. Angewandte Chemie, 136(1). https://doi.org/10.1002/ange.202312104

Wen, Xiaojin, Leopold, Viviane, & Seebeck, Florian P. (2024). Enzymatic synthesis of S -adenosyl- l -homocysteine and its nucleoside analogs from racemic homocysteine thiolactone. Chemical Science, 15(38), 15900–15906. https://doi.org/10.1039/d4sc03801k

Peng, Jiaming, Hughes, Gregory R., Müller, Manuel M., & Seebeck, Florian P. (2023). Enzymatic Fluoromethylation as a Tool for ATP‐Independent Ligation [Journal-article]. Angewandte Chemie International Edition, 63(1). https://doi.org/10.1002/anie.202312104

Reed, John H., & Seebeck, Florian P. (2023). Reagent Engineering for Group Transfer Biocatalysis [Journal-article]. Angewandte Chemie International Edition, 63(7). https://doi.org/10.1002/anie.202311159

Gericke, Lukas, Mhaindarkar, Dipali, Karst, Lukas C., Jahn, Sören, Kuge, Marco, Mohr, Michael K. F., Gagsteiger, Jana, Cornelissen, Nicolas V., Wen, Xiaojin, Mordhorst, Silja, Jessen, Henning J., Rentmeister, Andrea, Seebeck, Florian P., Layer, Gunhild, Loenarz, Christoph, & Andexer, Jennifer N. (2023). Biomimetic S-Adenosylmethionine Regeneration Starting from Multiple Byproducts Enables Biocatalytic Alkylation with Radical SAM Enzymes [Journal-article]. ChemBioChem, 24(9). https://doi.org/10.1002/cbic.202300133

Hartmann, Lea, Seebeck, Florian P., Schmalz, Hans-Günther, & Gründemann, Dirk. (2023). Isotope-labeled ergothioneine clarifies the mechanism of reaction with singlet oxygen [Journal-article]. Free Radical Biology and Medicine, 198, 12–26. https://doi.org/10.1016/j.freeradbiomed.2023.01.023

Beliaeva, Mariia A., Atac, Reyhan, & Seebeck, Florian P. (2022). Bacterial Degradation of Nτ-Methylhistidine. ACS Chemical Biology, 17(7), 1989–1995. https://doi.org/10.1021/acschembio.2c00437

Beliaeva, Mariia A., & Seebeck, Florian P. (2022). Discovery and Characterization of the Metallopterin-Dependent Ergothioneine Synthase from Caldithrix abyssi. JACS Au, 2(9), 2098–2107. https://doi.org/10.1021/jacsau.2c00365

Wen, Xiaojin, Leisinger, Florian, Leopold, Viviane, & Seebeck, Florian P. (2022). Synthetic Reagents for Enzyme-Catalyzed Methylation. Angewandte Chemie International Edition, 61(41), e202208746. https://doi.org/10.1002/anie.202208746

Beliaeva, Mariia A., Burn, Reto, Lim, David, & Seebeck , Florian P. (2021). In Vitro Production of Ergothioneine Isotopologues. Angewandte Chemie International Edition, 60(10), 5209–5212. https://doi.org/10.1002/anie.202011096

Beliaeva, Mariia A., Leisinger, Florian, & Seebeck, Florian P. (2021). In Vitro Reconstitution of a Five-Step Pathway for Bacterial Ergothioneine Catabolism. ACS Chemical Biology, 16(2), 397–403. https://doi.org/10.1021/acschembio.0c00968

Duncombe, Todd, Ponti, Aron, Seebeck, Florian Peter, & Dittrich, Petra. (2021). UV-Vis Spectra-Activated Droplet Sorting for Label-Free Chemical Identification and Collection of Drople. Analytical Chemistry, 93(38), 13008–13013. https://doi.org/10.1021/acs.analchem.1c02822

Leisinger, Florian, Miarzlou , Dzmitry A., & Seebeck, Florian P. (2021). Non-Coordinative Binding of O2 at the Active Center of a Copper-Dependent Enzyme. Angewandte Chemie International Edition, 60(11), 6154–6159. https://doi.org/10.1002/anie.202014981

Peng, Jiaming, Liao, Cangsong, Bauer, Carste, & Seebeck, Florian Peter. (2021). Fluorinated S-Adenosylmethionine as a Reagent for Enzyme-Catalyzed Fluoromethylation. Angewandte Chemie International Edition, 60(52), 27178–27183. https://doi.org/10.1002/anie.202108802

Schneider, Peter, Henßen, Birgit, Paschold, Beatrix, Chapple, Benjamin P., Schatton, Marcel, Seebeck, Florian Peter, Classen , Thomas, & Pietruszka, Jörg. (2021). Biocatalytic C3-Indole Methylation-A Useful Tool for the Natural-Product-Inspired Stereoselective Synthesis of Pyrroloindoles. Angewandte Chemie International Edition, 60(43), 23412–23418. https://doi.org/10.1002/anie.202107619

Flückger, Sebastian, Igareta, Nico V., & Seebeck, Florian P. (2020). Convergent Evolution of Fungal Cysteine Dioxygenases. ChemBioChem, 21(21), 3082–3086. https://doi.org/10.1002/cbic.202000317

Goncharenko, Kristina V., Flückiger, Sebastian, Liao, Cangsong, Lim, David, Stampfli, Anja R., & Seebeck, Florian P. (2020). Selenocysteine as a Substrate, an Inhibitor and a Mechanistic Probe for Bacterial and Fungal Iron-Dependent Sulfoxide Synthases. Chemistry - A European Journal, 26(6), 1328–1334. https://doi.org/10.1002/chem.201903898

Liao, Cangsong, & Seebeck, Florian P. (2020). Asymmetric β-Methylation of l- and d-α-Amino Acids by a Self-Contained Enzyme Cascade. Angewandte Chemie International Edition, 59(18), 7184–7187. https://doi.org/10.1002/anie.201916025

Lim, David, Wen, Xiaojin, & Seebeck, Florian P. (2020). Selenoimidazolium Salts as Supramolecular Reagents for Protein Alkylation. Chembiochem : A European Journal of Chemical Biology, 21(24), 3515–3520. https://doi.org/10.1002/cbic.202000557

Maurer, Alice, & Seebeck, Florian P. (2020). Reexamination of the Ergothioneine Biosynthetic Methyltransferase EgtD from Mycobacterium tuberculosis as a Protein Kinase Substrate. ChemBioChem, 21(20), 2908–2911. https://doi.org/10.1002/cbic.202000232

Milito, Alfonsina, Castellano, Immacolata, Burn, Reto, Seebeck, Florian P., Brunet, Christophe, & Palumbo, Anna. (2020). First evidence of ovothiol biosynthesis in marine diatoms. Free Radical Biology and Medicine, 152, 680–688. https://doi.org/10.1016/j.freeradbiomed.2020.01.010

Stampfli, Anja R., Blankenfeldt, Wulf, & Seebeck, Florian P. (2020). Structural basis of ergothioneine biosynthesis. Current Opinion in Structural Biology, 65, 1–8. https://doi.org/10.1016/j.sbi.2020.04.002

Stampfli, Anja R., & Seebeck, Florian P. (2020). The catalytic mechanism of sulfoxide synthases. Current Opinion in Chemical Biology, 59, 111–118. https://doi.org/10.1016/j.cbpa.2020.06.007

Leisinger, Florian, Burn, Reto, Meury, Marcel, Lukat, Peer, & Seebeck, Florian P. (2019). Structural and Mechanistic Basis for Anaerobic Ergothioneine Biosynthesis. Journal of the American Chemical Society, 141(17), 6906–6914. https://doi.org/10.1021/jacs.8b12596

Liao, Cangsong, & Seebeck, Florian P. (2019). S-adenosylhomocysteine as a methyl transfer catalyst in biocatalytic methylation reactions. Nature Catalysis, 2(8), 696–701. https://doi.org/10.1038/s41929-019-0300-0

Liao, Cangsong, & Seebeck, Florian Peter. (2019). In vitro reconstitution of bacterial DMSP biosynthesis. Angew. Chemie Int. Ed, 58(11), 3553–3556. https://doi.org/10.1002/anie.201814662

Lim, David, Gründemann, Dirk, & Seebeck, Florian P. (2019). Total Synthesis and Functional Characterization of Selenoneine. Angewandte Chemie International Edition, 58(42), 15026–15030. https://doi.org/10.1002/anie.201908967

Maurer, Alice, Leisinger, Florian, Lim, David, & Seebeck, Florian P. (2019). Structure and Mechanism of Ergothionase from Treponema denticola. Chemistry - A European Journal, 25(44), 10298–10303. https://doi.org/10.1002/chem.201901866

Miarzlou, Dzmitry A., Leisinger, Florian, Joss, Daniel, Häussinger, Daniel, & Seebeck, Florian P. (2019). Structure of formylglycine-generating enzyme in complex with copper and a substrate reveals an acidic pocket for binding and activation of molecular oxygen. Chemical Science, 10(29), 7049–7058. https://doi.org/10.1039/c9sc01723b

Stampfli, Anja R., Goncharenko, Kristina V., Meury, Marcel, Dubey, Badri N., Schirmer, Tilman, & Seebeck, Florian P. (2019). An Alternative Active Site Architecture for O2 Activation in the Ergothioneine Biosynthetic EgtB from Chloracidobacterium thermophilum. Journal of American Chemical Society, 141(13), 5275–5285. https://doi.org/10.1021/jacs.8b13023

Castellano, Immacolata, & Seebeck, Florian P. (2018). On ovothiol biosynthesis and biological roles: from life in the ocean to therapeutic potential. Natural Product Reports, 35(12), 1241–1250. https://doi.org/10.1039/c8np00045j

Gamage, Akshamal M., Liao, Cangsong, Cheah, Irwin K., Chen, Yahua, Lim, Daniel R. X., Ku, Joanne W. K., Chee, Rhonda Sin Ling, Gengenbacher, Martin, Seebeck, Florian P., Halliwell, Barry, & Gan, Yunn-Hwen. (2018). The proteobacterial species Burkholderia pseudomallei produces ergothioneine, which enhances virulence in mammalian infection. FASEB Journal, 32(12), 6395–6409. https://doi.org/10.1096/fj.201800716

Misson, Laëtitia, Burn, Reto, Vit, Allegra, Hildesheim, Julia, Beliaeva, Mariia A., Blankenfeldt, Wulf, & Seebeck, Florian P. (2018). Inhibition and Regulation of the Ergothioneine Biosynthetic Methyltransferase EgtD. ACS Chemical Biology, 13(5), 1333–1342. https://doi.org/10.1021/acschembio.8b00127

Burn, Reto, Misson, Laëtitia, Meury, Marcel, & Seebeck, Florian P. (2017). Anaerobic Origin of Ergothioneine. Angewandte Chemie International Edition, 56(41), 12508–12511. https://doi.org/10.1002/anie.201705932

Faponle, Abayomi S., Seebeck, Florian P., & de Visser, Sam P. (2017). Sulfoxide Synthase versus Cysteine Dioxygenase Reactivity in a Nonheme Iron Enzyme. Journal of the American Chemical Society, 139(27), 9259–9270. https://doi.org/10.1021/jacs.7b04251

Knop, Matthias, Dang, Thanh Quy, Jeschke, Gunnar, & Seebeck, Florian P. (2017). Copper is a Cofactor of the Formylglycine-Generating Enzyme. ChemBioChem, 18(2), 161–165. https://doi.org/10.1002/cbic.201600359

Knop, Matthias, Lemnaru, Roxana, & Seebeck, Florian P. (2017). Mutation of Conserved Residues Increases in Vitro Activity of the Formylglycine-Generating Enzyme. ChemBioChem, 18(17), 1755–1761. https://doi.org/10.1002/cbic.201700174

Liao, Cangsong, & Seebeck, Florian P. (2017). Convergent Evolution of Ergothioneine Biosynthesis in Cyanobacteria. ChemBioChem, 18(21), 2115–2118. https://doi.org/10.1002/cbic.201700354

Meury, Marcel, Knop, Matthias, & Seebeck, Florian P. (2017). Structural Basis for Copper-Oxygen Mediated C-H Bond Activation by the Formylglycine-Generating Enzyme. Angewandte Chemie International Edition, 56(28), 8115–8119. https://doi.org/10.1002/anie.201702901

Goncharenko, Kristina V, & Seebeck, Florian P. (2016). Conversion of a non-heme iron-dependent sulfoxide synthase into a thiol dioxygenase by a single point mutation. Chemical Communications, 52(9), 8–1945. https://doi.org/10.1039/c5cc07772a

Goncharenko, Kristina V., Vit, Allegra, Blankenfeldt, Wulf, & Seebeck, Florian P. (2015). Structure of the Sulfoxide Synthase EgtB from the Ergothioneine Biosynthetic Pathway. Angewandte Chemie International Edition, 54(9), 4–2821. https://doi.org/10.1002/anie.201410045

Knop, Matthias, Engi, Pascal, Lemnaru, Roxana, & Seebeck, Florian P. (2015). In Vitro Reconstitution of Formylglycine-Generating Enzymes Requires Copper(I). ChemBioChem, 16(15), 2147–2150. https://doi.org/10.1002/cbic.201500322

Vit, Allegra, Mashabela, Gabriel, Blankenfeldt, Wulf, & Seebeck, Florian P. (2015). Structure of the Ergothioneine-Biosynthesis Amidohydrolase EgtC. ChemBioChem, 16(10), 6–1490. https://doi.org/10.1002/cbic.201500168

Vit, Allegra, Misson, L., Blankenfeldt, W., & Seebeck, Florian P. (2015). Ergothioneine Biosynthetic Methyltransferase EgtD Reveals the Structural Basis of Aromatic Amino Acid Betaine Biosynthesis. ChemBioChem, 16(1), 25–119. https://doi.org/10.1002/cbic.201402522

Vit, Allegra, Misson, Laetitia, Blankenfeldt, W., & Seebeck, Florian. (2014). Crystallization and preliminary X-ray analysis of the ergothioneine-biosynthetic methyltransferase EgtD. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 70(Pt 5), 80–676. https://doi.org/10.1107/s2053230x1400805x

Mashabela, Gabriel T M, & Seebeck, Florian P. (2013). Substrate specificity of an oxygen dependent sulfoxide synthase in ovothiol biosynthesis. Chemical Communications, 49(70), 6–7714. https://doi.org/10.1039/c3cc42594k

Seebeck, Florian. (2013). Thiohistidine biosynthesis. Chimia, 67(5), 333–336. https://doi.org/10.2533/chimia.2013.333

Amrein, Beat, Schmid, Maurus, Collet, Guillaume, Cuniasse, Philippe, Gilardoni, Francois, Seebeck, Florian P., & Ward, Thomas R. (2012). Identification of two-histidines one-carboxylate binding motifs in proteins amenable to facial coordination to metals. Metallomics, 4(4), 88–379. https://doi.org/10.1039/c2mt20010d

Hofmann, Frank T., Szostak, Jack W., & Seebeck, Florian P. (2012). In vitro selection of functional lantipeptides. Journal of the American Chemical Society, 134(19), 8038–8041. https://doi.org/10.1021/ja302082d