[FG] Huwyler JörgHead of Research Unit Prof. Dr.Jörg HuwylerOverviewMembersPublicationsProjects & CollaborationsProjects & Collaborations OverviewMembersPublicationsProjects & Collaborations Projects & Collaborations 2 foundShow per page10 10 20 50 Hepatotoxicity (part of SCAHT Research Projects 2013 - 2016) Research Project | 4 Project MembersThe project has three parts, which are started simultaneously since different research groups are involved in the project. Part 1 consists of the establishment of suitable cell models for performing toxicological studies with hepatocytes. We are going to add HepaRG cells to already existing HepG2 and HUH cell lines. In addition, we are going to characterize 3-dimensional human hepatocytes. We are also going to prepare hepatocytes containing genes enabling to detect ROS generation in different cellular compartments. Part 2 describes the application of these systems and analytical techniques for studying diverse known hepatotoxins. The idea is to find out mechanisms of toxicity, proof these mechanisms in cellular systems, find out potential susceptibility factors and proof such factors in genetically manipulated cells and in experimental animals. If suitable, such susceptibility factors can also be tested using a large tissue biobank and/or in cohorts of patients with drug-induced induced liver injury. In Part 3, the cellular uptake and the toxicity of nanoparticles will be tested in our established hepatocyte systems and in experimental animals. Mechanism of action of MDMA (Ecstasy) Research Project | 7 Project MembersIn our main project: "Mechanism of action of MDMA (Ecstasy)" we investigate how MDMA acts in the brain using both in vitro techniques and studies in humans. In the in vitro studies we assess interactions of MDMA with serotonin-, dopamine-, and norepinephrine uptake carriers in cell assays. In humans, we assess the subjective and cardiovascular effects of MDMA after the administration of substances that selectively block potential sites of action of MDMA in the brain. 1 1 OverviewMembersPublicationsProjects & Collaborations
Projects & Collaborations 2 foundShow per page10 10 20 50 Hepatotoxicity (part of SCAHT Research Projects 2013 - 2016) Research Project | 4 Project MembersThe project has three parts, which are started simultaneously since different research groups are involved in the project. Part 1 consists of the establishment of suitable cell models for performing toxicological studies with hepatocytes. We are going to add HepaRG cells to already existing HepG2 and HUH cell lines. In addition, we are going to characterize 3-dimensional human hepatocytes. We are also going to prepare hepatocytes containing genes enabling to detect ROS generation in different cellular compartments. Part 2 describes the application of these systems and analytical techniques for studying diverse known hepatotoxins. The idea is to find out mechanisms of toxicity, proof these mechanisms in cellular systems, find out potential susceptibility factors and proof such factors in genetically manipulated cells and in experimental animals. If suitable, such susceptibility factors can also be tested using a large tissue biobank and/or in cohorts of patients with drug-induced induced liver injury. In Part 3, the cellular uptake and the toxicity of nanoparticles will be tested in our established hepatocyte systems and in experimental animals. Mechanism of action of MDMA (Ecstasy) Research Project | 7 Project MembersIn our main project: "Mechanism of action of MDMA (Ecstasy)" we investigate how MDMA acts in the brain using both in vitro techniques and studies in humans. In the in vitro studies we assess interactions of MDMA with serotonin-, dopamine-, and norepinephrine uptake carriers in cell assays. In humans, we assess the subjective and cardiovascular effects of MDMA after the administration of substances that selectively block potential sites of action of MDMA in the brain. 1 1
Hepatotoxicity (part of SCAHT Research Projects 2013 - 2016) Research Project | 4 Project MembersThe project has three parts, which are started simultaneously since different research groups are involved in the project. Part 1 consists of the establishment of suitable cell models for performing toxicological studies with hepatocytes. We are going to add HepaRG cells to already existing HepG2 and HUH cell lines. In addition, we are going to characterize 3-dimensional human hepatocytes. We are also going to prepare hepatocytes containing genes enabling to detect ROS generation in different cellular compartments. Part 2 describes the application of these systems and analytical techniques for studying diverse known hepatotoxins. The idea is to find out mechanisms of toxicity, proof these mechanisms in cellular systems, find out potential susceptibility factors and proof such factors in genetically manipulated cells and in experimental animals. If suitable, such susceptibility factors can also be tested using a large tissue biobank and/or in cohorts of patients with drug-induced induced liver injury. In Part 3, the cellular uptake and the toxicity of nanoparticles will be tested in our established hepatocyte systems and in experimental animals.
Mechanism of action of MDMA (Ecstasy) Research Project | 7 Project MembersIn our main project: "Mechanism of action of MDMA (Ecstasy)" we investigate how MDMA acts in the brain using both in vitro techniques and studies in humans. In the in vitro studies we assess interactions of MDMA with serotonin-, dopamine-, and norepinephrine uptake carriers in cell assays. In humans, we assess the subjective and cardiovascular effects of MDMA after the administration of substances that selectively block potential sites of action of MDMA in the brain.
