Molecular Bionics (Seebeck)

Jiang, Y., Li, Z. and Li, S. (2025) ‘Superoxide-mediated O2 activation drives radical cyclization in ergot alkaloid biosynthesis’, Engineering Microbiology, 5(2). Available at: https://doi.org/10.1016/j.engmic.2025.100207.

Jiang, Y. et al. (2025) ‘Unexpected Activities of CYP152 Peroxygenases towards Non-carboxylic Substrates Reveal Novel Substrate Recognition Mechanism and Catalytic Versatility’, Angewandte Chemie - International Edition [Preprint]. Available at: https://doi.org/10.1002/anie.202506614.

Peng, J. (2025) Bionic alkyltransferases for biocatalysis. Doctoral Thesis.

Li, S. et al. (2024) ‘Functional analysis of the whole CYPome and Fdxome of Streptomyces venezuelae ATCC 15439’, Engineering Microbiology, 4(4). Available at: https://doi.org/10.1016/j.engmic.2024.100166.

Jiang, Y. et al. (2024) ‘Photoenzymatic synthesis of 1-alkenes and hydroxyl fatty acids by cascading a COF photocatalyst and P450 peroxygenases’, Science Bulletin, 69(21), pp. 3350–3354. Available at: https://doi.org/10.1016/j.scib.2024.04.069.

Nalivaiko, Egor Y. and Seebeck, Florian P. (2024) ‘A Rhodanese‐Like Enzyme that Catalyzes Desulfination of Ergothioneine Sulfinic Acid’, ChemBioChem. 02.05.2024, 25(9). Available at: https://doi.org/10.1002/cbic.202400131.

Vasseur, Camille M., Karunasegaram, Dishani and Seebeck, Florian P. (2024) ‘Structure and Substrate Specificity of S -Methyl Thiourocanate Hydratase’, ACS Chemical Biology. 22.02.2024, 19(3), pp. 718–724. Available at: https://doi.org/10.1021/acschembio.3c00745.

Nalivaiko, Egor Y., Vasseur, Camille M. and Seebeck, Florian P. (2024) ‘Enzyme‐Catalyzed Oxidative Degradation of Ergothioneine’, Angewandte Chemie International Edition. 19.02.2024, 63(8). Available at: https://doi.org/10.1002/anie.202318445.

Peng, Jiaming et al. (2024) ‘Enzymatic Fluoromethylation as a Tool for ATP‐Independent Ligation’, Angewandte Chemie. 02.01.2024, 136(1). Available at: https://doi.org/10.1002/ange.202312104.

Carrillo, M. (2024) Polymer fixed-targets for time-resolved serial protein crystallography at XFELs and synchrotrons. Doctoral Thesis.

F. M . de Carvalho, C. (2024) Isotopic fractionation of molecular oxygen during enzymatic O2-consuming processes in aquatic environments. Doctoral Thesis.

Wen, X. (2024) Synthetic reagents for in vitro enzymatic and protein alkylation. Doctoral Thesis.

Wen, Xiaojin, Leopold, Viviane and Seebeck, Florian P. (2024) ‘Enzymatic synthesis of S -adenosyl- l -homocysteine and its nucleoside analogs from racemic homocysteine thiolactone’, Chemical Science. 06.09.2024, 15(38), pp. 15900–15906. Available at: https://doi.org/10.1039/d4sc03801k.

Peng, Jiaming et al. (2023) ‘Enzymatic Fluoromethylation as a Tool for ATP‐Independent Ligation’, Angewandte Chemie International Edition. 13.11.2023, 63(1). Available at: https://doi.org/10.1002/anie.202312104.

Reed, John H. and Seebeck, Florian P. (2023) ‘Reagent Engineering for Group Transfer Biocatalysis’, Angewandte Chemie International Edition. 09.09.2023, 63(7). Available at: https://doi.org/10.1002/anie.202311159.

Gericke, Lukas et al. (2023) ‘Biomimetic S-Adenosylmethionine Regeneration Starting from Multiple Byproducts Enables Biocatalytic Alkylation with Radical SAM Enzymes’, ChemBioChem, 24(9). Available at: https://doi.org/10.1002/cbic.202300133.

Hartmann, Lea et al. (2023) ‘Isotope-labeled ergothioneine clarifies the mechanism of reaction with singlet oxygen’, Free Radical Biology and Medicine. 02.02.2023, 198, pp. 12–26. Available at: https://doi.org/10.1016/j.freeradbiomed.2023.01.023.

Beliaeva, M. (2022) The enzymatic landscape of ergothioneine degradation. Doctoral Thesis.

Beliaeva, Mariia A., Atac, Reyhan and Seebeck, Florian P. (2022) ‘Bacterial Degradation of Nτ-Methylhistidine’, ACS Chemical Biology, 17(7), pp. 1989–1995. Available at: https://doi.org/10.1021/acschembio.2c00437.

Beliaeva, Mariia A. and Seebeck, Florian P. (2022) ‘Discovery and Characterization of the Metallopterin-Dependent Ergothioneine Synthase from Caldithrix abyssi’, JACS Au, 2(9), pp. 2098–2107. Available at: https://doi.org/10.1021/jacsau.2c00365.

Igareta, N.V. (2022) Expanding the secondary coordination sphere of streptavidin-based artificial metalloenzymes and their characterization . Doctoral Thesis.

Leisinger, F. (2022) How structure guides function: ergothioneine anabolism and catabolism formylglycine generating enzyme methyl transferases. Doctoral Thesis.

Wen, Xiaojin et al. (2022) ‘Synthetic Reagents for Enzyme-Catalyzed Methylation’, Angewandte Chemie International Edition, 61(41), p. e202208746. Available at: https://doi.org/10.1002/anie.202208746.

Beliaeva, Mariia A. et al. (2021) ‘In Vitro Production of Ergothioneine Isotopologues’, Angewandte Chemie International Edition, 60(10), pp. 5209–5212. Available at: https://doi.org/10.1002/anie.202011096.

Beliaeva, Mariia A., Leisinger, Florian and Seebeck, Florian P. (2021) ‘In Vitro Reconstitution of a Five-Step Pathway for Bacterial Ergothioneine Catabolism’, ACS Chemical Biology, 16(2), pp. 397–403. Available at: https://doi.org/10.1021/acschembio.0c00968.

Burn, R. (2021) Enzymatic strategies for carbon-sulfur bond formation in ergothioneine biosynthesis. Doctoral Thesis.

Duncombe, Todd et al. (2021) ‘UV-Vis Spectra-Activated Droplet Sorting for Label-Free Chemical Identification and Collection of Drople’, Analytical Chemistry, 93(38), pp. 13008–13013. Available at: https://doi.org/10.1021/acs.analchem.1c02822.

Leisinger, Florian, Miarzlou , Dzmitry A. and Seebeck, Florian P. (2021) ‘Non-Coordinative Binding of O2 at the Active Center of a Copper-Dependent Enzyme’, Angewandte Chemie International Edition, 60(11), pp. 6154–6159. Available at: https://doi.org/10.1002/anie.202014981.

Miarzlou, D. (2021) Insights into the mechanism and structure of formylglycine generating enzyme. Doctoral Thesis.

Peng, Jiaming et al. (2021) ‘Fluorinated S-Adenosylmethionine as a Reagent for Enzyme-Catalyzed Fluoromethylation’, Angewandte Chemie International Edition, 60(52), pp. 27178–27183. Available at: https://doi.org/10.1002/anie.202108802.

Schneider, Peter et al. (2021) ‘Biocatalytic C3-Indole Methylation-A Useful Tool for the Natural-Product-Inspired Stereoselective Synthesis of Pyrroloindoles’, Angewandte Chemie International Edition, 60(43), pp. 23412–23418. Available at: https://doi.org/10.1002/anie.202107619.

Vallapurackal, J. (2021) Development of a Microfluidics-Based Screening Assay for the High-Throughput Directed Evolution of Artificial Metalloenzymes . Doctoral Thesis.

Flückger, Sebastian, Igareta, Nico V. and Seebeck, Florian P. (2020) ‘Convergent Evolution of Fungal Cysteine Dioxygenases’, ChemBioChem, 21(21), pp. 3082–3086. Available at: https://doi.org/10.1002/cbic.202000317.

Goncharenko, Kristina V. et al. (2020) ‘Selenocysteine as a Substrate, an Inhibitor and a Mechanistic Probe for Bacterial and Fungal Iron-Dependent Sulfoxide Synthases’, Chemistry - A European Journal, 26(6), pp. 1328–1334. Available at: https://doi.org/10.1002/chem.201903898.

Liao, Cangsong and Seebeck, Florian P. (2020) ‘Asymmetric β-Methylation of l- and d-α-Amino Acids by a Self-Contained Enzyme Cascade’, Angewandte Chemie International Edition, 59(18), pp. 7184–7187. Available at: https://doi.org/10.1002/anie.201916025.

Lim, David, Wen, Xiaojin and Seebeck, Florian P. (2020) ‘Selenoimidazolium Salts as Supramolecular Reagents for Protein Alkylation’, Chembiochem : a European journal of chemical biology, 21(24), pp. 3515–3520. Available at: https://doi.org/10.1002/cbic.202000557.

Maurer, A. (2020) The Mechanism of Enzyme-catalyzed Ergothioneine Degradation. Doctoral Thesis.

Maurer, Alice and Seebeck, Florian P. (2020) ‘Reexamination of the Ergothioneine Biosynthetic Methyltransferase EgtD from Mycobacterium tuberculosis as a Protein Kinase Substrate’, ChemBioChem, 21(20), pp. 2908–2911. Available at: https://doi.org/10.1002/cbic.202000232.

Milito, Alfonsina et al. (2020) ‘First evidence of ovothiol biosynthesis in marine diatoms’, Free radical biology and medicine, 152, pp. 680–688. Available at: https://doi.org/10.1016/j.freeradbiomed.2020.01.010.

Stampfli, Anja R., Blankenfeldt, Wulf and Seebeck, Florian P. (2020) ‘Structural basis of ergothioneine biosynthesis’, Current Opinion in Structural Biology, 65, pp. 1–8. Available at: https://doi.org/10.1016/j.sbi.2020.04.002.

Stampfli, Anja R. and Seebeck, Florian P. (2020) ‘The catalytic mechanism of sulfoxide synthases’, Current Opinion in Chemical Biology, 59, pp. 111–118. Available at: https://doi.org/10.1016/j.cbpa.2020.06.007.

Di Girolamo, S. (2019) First characterization of a class F sortase and establishment of a microreactor-based assay for its directed evolution. Doctoral Thesis. Available at: https://doi.org/10.5451/unibas-007215325.

Engi, P. (2019) Chemical probes for mechanistic enzymology. Doctoral Thesis. Available at: https://doi.org/10.5451/unibas-007116162.

Leisinger, Florian et al. (2019) ‘Structural and Mechanistic Basis for Anaerobic Ergothioneine Biosynthesis’, Journal of the American Chemical Society, 141(17), pp. 6906–6914. Available at: https://doi.org/10.1021/jacs.8b12596.

Liao, Cangsong and Seebeck, Florian P. (2019) ‘S-adenosylhomocysteine as a methyl transfer catalyst in biocatalytic methylation reactions’, Nature Catalysis, 2(8), pp. 696–701. Available at: https://doi.org/10.1038/s41929-019-0300-0.

Liao, Cangsong and Seebeck, Florian Peter (2019) ‘In vitro reconstitution of bacterial DMSP biosynthesis’, Angew. Chemie Int. Ed, 58(11), pp. 3553–3556. Available at: https://doi.org/10.1002/anie.201814662.

Lim, David, Gründemann, Dirk and Seebeck, Florian P. (2019) ‘Total Synthesis and Functional Characterization of Selenoneine’, Angewandte Chemie International Edition, 58(42), pp. 15026–15030. Available at: https://doi.org/10.1002/anie.201908967.

Maurer, Alice et al. (2019) ‘Structure and Mechanism of Ergothionase from Treponema denticola’, Chemistry - A European Journal, 25(44), pp. 10298–10303. Available at: https://doi.org/10.1002/chem.201901866.

Miarzlou, Dzmitry A. et al. (2019) ‘Structure of formylglycine-generating enzyme in complex with copper and a substrate reveals an acidic pocket for binding and activation of molecular oxygen’, Chemical Science, 10(29), pp. 7049–7058. Available at: https://doi.org/10.1039/c9sc01723b.

Stampfli, A.R. (2019) The Diversity of the Iron-dependent Sulfoxide Synthases in Ergothioneine Biosynthesis. Doctoral Thesis.

Stampfli, Anja R. et al. (2019) ‘An Alternative Active Site Architecture for O2 Activation in the Ergothioneine Biosynthetic EgtB from Chloracidobacterium thermophilum’, Journal of American Chemical Society, 141(13), pp. 5275–5285. Available at: https://doi.org/10.1021/jacs.8b13023.

Castellano, Immacolata and Seebeck, Florian P. (2018) ‘On ovothiol biosynthesis and biological roles: from life in the ocean to therapeutic potential’, Natural Product Reports, 35(12), pp. 1241–1250. Available at: https://doi.org/10.1039/c8np00045j.

Flückiger, S. (2018) Mechanistic studies of sulfur-carbon bond formation by metal-dependent enzymes. Doctoral Thesis. Available at: https://doi.org/10.5451/unibas-007178301.

Gamage, Akshamal M. et al. (2018) ‘The proteobacterial species Burkholderia pseudomallei produces ergothioneine, which enhances virulence in mammalian infection’, FASEB Journal, 32(12), pp. 6395–6409. Available at: https://doi.org/10.1096/fj.201800716.

Misson, Laëtitia et al. (2018) ‘Inhibition and Regulation of the Ergothioneine Biosynthetic Methyltransferase EgtD’, ACS Chemical Biology, 13(5), pp. 1333–1342. Available at: https://doi.org/10.1021/acschembio.8b00127.

Burn, Reto et al. (2017) ‘Anaerobic Origin of Ergothioneine’, Angewandte Chemie International Edition, 56(41), pp. 12508–12511. Available at: https://doi.org/10.1002/anie.201705932.

Faponle, Abayomi S., Seebeck, Florian P. and de Visser, Sam P. (2017) ‘Sulfoxide Synthase versus Cysteine Dioxygenase Reactivity in a Nonheme Iron Enzyme’, Journal of the American Chemical Society, 139(27), pp. 9259–9270. Available at: https://doi.org/10.1021/jacs.7b04251.

Goncharenko, K. (2017) The catalytic mechanism of the iron-dependent sulfoxide synthase EgtB. Doctoral Thesis. Available at: https://doi.org/10.5451/unibas-007085306.

Knop, M. (2017) Identification and characterization of a novel copper dependent enzyme. Doctoral Thesis. Available at: https://doi.org/10.5451/unibas-006736737.

Knop, Matthias et al. (2017) ‘Copper is a Cofactor of the Formylglycine-Generating Enzyme’, ChemBioChem, 18(2), pp. 161–165. Available at: https://doi.org/10.1002/cbic.201600359.

Knop, Matthias, Lemnaru, Roxana and Seebeck, Florian P. (2017) ‘Mutation of Conserved Residues Increases in Vitro Activity of the Formylglycine-Generating Enzyme.’, ChemBioChem, 18(17), pp. 1755–1761. Available at: https://doi.org/10.1002/cbic.201700174.

Liao, Cangsong and Seebeck, Florian P. (2017) ‘Convergent Evolution of Ergothioneine Biosynthesis in Cyanobacteria’, ChemBioChem, 18(21), pp. 2115–2118. Available at: https://doi.org/10.1002/cbic.201700354.

Meury, Marcel, Knop, Matthias and Seebeck, Florian P. (2017) ‘Structural Basis for Copper-Oxygen Mediated C-H Bond Activation by the Formylglycine-Generating Enzyme’, Angewandte Chemie International Edition, 56(28), pp. 8115–8119. Available at: https://doi.org/10.1002/anie.201702901.

Goncharenko, Kristina V and Seebeck, Florian P (2016) ‘Conversion of a non-heme iron-dependent sulfoxide synthase into a thiol dioxygenase by a single point mutation.’, Chemical Communications, 52(9), pp. 1945–8. Available at: https://doi.org/10.1039/c5cc07772a.

Lemnaru, Roxana (2016) A chemoenzymatic strategy for protein-nanocellulose conjugates. Dissertation. Universität Basel.

Lemnaru, R. (2016) A chemoenzymatic strategy for protein-nanocellulose conjugates. Doctoral Thesis. Available at: https://doi.org/10.5451/unibas-006856826.

Misson, Laetitia (2016) Insights into the mechansims and regluation of EgtD, a novel histidine methyltransferase from ergothioneine biosynthesis. Dissertation. Universität Basel.

Misson, L. (2016) Insights into the mechanism and regulation of EgtD, a novel histidine methyltransferase from ergothioneine biosynthesis. Doctoral Thesis. Available at: https://doi.org/10.5451/unibas-006683670.

Goncharenko, Kristina V. et al. (2015) ‘Structure of the Sulfoxide Synthase EgtB from the Ergothioneine Biosynthetic Pathway’, Angewandte Chemie International Edition, 54(9), pp. 2821–4. Available at: https://doi.org/10.1002/anie.201410045.

Knop, Matthias et al. (2015) ‘In Vitro Reconstitution of Formylglycine-Generating Enzymes Requires Copper(I)’, ChemBioChem, 16(15), pp. 2147–2150. Available at: https://doi.org/10.1002/cbic.201500322.

Vit, Allegra et al. (2015) ‘Structure of the Ergothioneine-Biosynthesis Amidohydrolase EgtC’, ChemBioChem, 16(10), pp. 1490–6. Available at: https://doi.org/10.1002/cbic.201500168.

Vit, Allegra et al. (2015) ‘Ergothioneine Biosynthetic Methyltransferase EgtD Reveals the Structural Basis of Aromatic Amino Acid Betaine Biosynthesis’, ChemBioChem, 16(1), pp. 119–25. Available at: https://doi.org/10.1002/cbic.201402522.

Vit, Allegra et al. (2014) ‘Crystallization and preliminary X-ray analysis of the ergothioneine-biosynthetic methyltransferase EgtD’, Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 70(Pt 5), pp. 676–80. Available at: https://doi.org/10.1107/s2053230x1400805x.

Mashabela, Gabriel T M and Seebeck, Florian P (2013) ‘Substrate specificity of an oxygen dependent sulfoxide synthase in ovothiol biosynthesis’, Chemical Communications, 49(70), pp. 7714–6. Available at: https://doi.org/10.1039/c3cc42594k.

Seebeck, Florian (2013) ‘Thiohistidine biosynthesis’, Chimia, 67(5), pp. 333–336. Available at: https://doi.org/10.2533/chimia.2013.333.

Hofmann, Frank T., Szostak, Jack W. and Seebeck, Florian P. (2012) ‘In vitro selection of functional lantipeptides’, Journal of the American Chemical Society, 134(19), pp. 8038–8041. Available at: https://doi.org/10.1021/ja302082d.